The hypothalamus-pituitary-adrenal (HPA) axis is activated in response to inflammation leading to increased production of anti-inflammatory glucocorticoids by the adrenal cortex, thereby representing an endogenous feedback loop. However, severe inflammation reduces the responsiveness of the adrenal gland to adrenocorticotropic hormone (ACTH), although the underlying mechanisms are poorly understood. Here, we show by transcriptomic, proteomic, and metabolomic analyses that LPS-induced systemic inflammation triggers profound metabolic changes in steroidogenic adrenocortical cells, including downregulation of the TCA cycle and oxidative phosphorylation, in mice. Inflammation disrupts the TCA cycle at the level of succinate dehydrogenase (SDH), leading to succinate accumulation and disturbed steroidogenesis. Mechanistically, IL-1β reduces SDHB expression through upregulation of DNA methyltransferase 1 (DNMT1) and methylation of the SDHB promoter. Consequently, increased succinate levels impair oxidative phosphorylation and ATP synthesis and enhance ROS production, leading to reduced steroidogenesis. Together, we demonstrate that the IL-1β-DNMT1-SDHB-succinate axis disrupts steroidogenesis. Our findings not only provide a mechanistic explanation for adrenal dysfunction in severe inflammation, but also offer a potential target for therapeutic intervention.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:92915 |
| Date | 19 August 2024 |
| Creators | Mateska, Ivona, Witt, Anke, Hagag, Eman, Sinha, Anupam, Yilmaz, Canelif, Thanou, Evangelia, Sun, Na, Kolliniati, Ourania, Patschin, Maria, Abdelmegeed, Heba, Henneicke, Holger, Kanczkowski, Waldemar, Wielockx, Ben, Tsatsanis, Christos, Dahl, Andreas, Walch, Axel Karl, Li, Ka Wan, Peitzsch, Mirko, Chavakis, Triantafyllos, Alexaki, Vasileia Ismini |
| Publisher | eLife Sciences Publications |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2050-084X, 83064, 10.7554/eLife.83064, info:eu-repo/grantAgreement/European Commission/H2020 | MSCA-ITN-ETN/765704//A European training network for the discovery of neurotrophins small molecule mimetics as candidate therapeutic agents for neurodegeneration and neuroinflammation/EuroNeurotrophin, info:eu-repo/grantAgreement/Deutsche Forschungsgemeinschaft/TRR 205: Die Nebenniere: Zentrales Relais in Gesundheit und Krankheit/314061271/ |
Page generated in 0.0031 seconds