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Previous issue date: 2018-03-08 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O uso de plantas medicinais ? uma pr?tica comum da humanidade desde a antiguidade. Tal conhecimento baseado na experi?ncia tem sido repassado por muitas comunidades e grupos ?tnicos, e serve como ponto de partida para estudos bioqu?micos e farmacol?gicos dos princ?pios ativos presentes nas plantas. Para isto, neste trabalho foram avaliados o potencial antioxidante e tamb?m biol?gico, utilizando sistemas in vitro e in vivo dos diferentes extratos obtidos de folhas da planta Coccoloba alnifolia. Foram obtidos inicialmente seis extratos: hexano (EH), clorof?rmio (EC), etan?lico (EE), metan?lico (EM), ?gua final (EAF) e aquoso (EA) a partir das folhas frescas da planta. Posteriormente, foram avaliados o teor de a??car, prote?nas e compostos fen?licos. Os resultados obtidos na triagem fitoqu?mica sugerem a presen?a de fen?is, flavonoides, saponinas, n?cleo triterp?nicos e esteroidais e n?cleo esteroidal insaturado, j? com a metodologia de cromatografia em camada delgada (CCD), foi observado uma abund?ncia de flavonoides. Foram realizados quatro ensaios: Teste do Poder Redutor, Quela??o de ?ons Metais, Quela??o de Cobre e Atividade Sequestradora de Radical Hidroxila. Estes ensaios permitiram verificar que os extratos apresentaram atividade antioxidante nas concentra??es avaliadas de 100, 250 e 500 ?g/mL. Foi verificado principalmente para os extratos EE, EM e EA as atividades antioxidante nos ensaios poder redutor e sequestro do radical hidroxila. A correla??o de Pearson mostrou que estes dados est?o associados aos compostos fen?licos e a??cares. E com o modelo das linhagens celulares foi observado que a maioria dos extratos avaliados neste trabalho n?o mostrou-se citot?xico para as linhagens celulares. Os extratos tamb?m n?o inibiram o crescimento da levedura Candida parapsilosis. Os extratos obtidos da folha de C. alnifolia apresenta um potencial antioxidante interessante, e os resultados obtidos tanto nos modelos in vitro, como as linhagens celulares, refor?am que s?o necess?rios mais estudos para compreender o potencial destes extratos in vivo e identificar quais s?o as biomol?culas associadas com estas atividades e seus respectivos potenciais biotecnol?gicos. / The use of medicinal plants has been a common practice in folk medicine. This knowledge has been based on the experience that it has been passed though many generations. However, many times this knowledge do not have any scientific base. The molecules responsive for these activities are the secondary metabolic produce as a response to environmental conditions (biotic or abiotic stress). The Mata atl?ntica biome has a rich flora, which needs to be studied for its biological activities, and its potential to identify new biomolecules. In order to do this, six extracts (HE, CE, EE, ME, WEE e WE) were obtained from Coccoloba alnifolia leaves and then it was evaluated the antioxidant and the biological potential using different in vitro and in vivo systems. First, it was measured the sugar content, proteins and phenolic compounds. Based on these results, qualitative experiments were carried out to determine the secondary metabolites present in these extracts. It was detected the presence of phenols, flavonoids, saponins, triterpenic, and steroidal nuclei and unsaturated steroidal nuclei. After that, it was evaluated the antioxidant potential by four in vitro assay: Redution Power test, Metal Ion Chelation, Copper Chelation and Hydroxyl Radical Scavenging Activity. These assays allowed us verify that the extracts presented antioxidant activity at extract concentrations: 100, 250 and 500 ?g/mL. It was observed the ethanolic, methanolic and aqueous extracts had activity in reducing power and scavenging of the hydroxyl radical. The Pearson?s correlation showed that these results may be associated to the presence of phenolic compounds and sugars. The third in vitro model used in this work was cell culture. When it was used the 3T3 lineage it was observed that these extracts were not cytotoxic. However, it was not observed any antiproliferative activity in tumor cell lineages. In sumary, the data obtained here showed that using in vitro assays that these extracts in special ethanolic and aqueous had an interesting antioxidant potential and it will be important to go further in this research.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/24994 |
| Date | 08 March 2018 |
| Creators | Melo, Luciana Fentanes Moura de |
| Contributors | 13451112825, Santos, Deborah Yara Alves Cursino dos, 10641608837, Lima, Jailma Almeida de, 05116158411, Rocha, Hugo Alexandre de Oliveira, Scortecci, Katia Castanho |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM BIOQU?MICA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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