Type 1 diabetes is an autoimmune disease leading to insulin deficiency. Autoantibodies to beta cell proteins are already present in the asymptomatic phase of type 1 diabetes. Recent findings have suggested a number of additional minor autoantigens in patients with type 1 diabetes. We have established luciferase immunoprecipitation systems (LIPS) for anti-MTIF3, anti-PPIL2, anti-NUP50 and anti-MLH1 and analyzed samples from 500 patients with type 1 diabetes at onset of clinical disease and 200 healthy individuals who had a family
history of type 1 diabetes but no evidence of beta cell autoantibodies. We show significantly higher frequencies of anti-MTIF3, anti-PPIL2 and anti-MLH1 in recent onset type 1 diabetes patients in comparison to controls. In addition, antibodies to NUP50 were associated with HLA-DRB1*03 and antibodies to MLH1 were associated with HLA-DRB1*04 genotypes.:1. Introduction
2. Material and methods
2.1 Participants
2.2. Cloning and expression of antigens
2.3. Luciferase ImmunoPrecipitation (LIPS) assays
2.4. Antinuclear antibodies (ANA)
2.5. Statistics
3. Results
4. Discussion
5. Conclusion
Declaration of interest
Funding
Acknowledgements
Appendix A Suplementary data
References
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:33337 |
| Date | 27 February 2019 |
| Creators | Bonifacio, Ezio, Müller, Denise, Telieps, Tanja, Eugster, Anne, Weinzierl, Christina, Jolink, Manja, Ziegler, Anette-Gabriele |
| Contributors | Clinical Immunology - Elsevier, Technische Universität Dresden - DFG Research Center for Regenerative Therapeis Dresden |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 10.1016/j.clim.2018.07.001, 1521-7035, 10.1097/PAS.0000000000000949 |
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