In the heart, unidirectional blood flow depends on proper heart valve function. As, in mammals, regulatory mechanisms
of early heart valve and bone development are shown to contribute to adult heart valve pathologies, we used the animal
model zebrafish (ZF, Danio rerio) to investigate the microarchitecture and differentiation of cardiac valve interstitial
cells in the transition from juvenile (35 days) to end of adult breeding (2.5 years) stages. Of note, light microscopy
and immunohistochemistry revealed major differences in ZF heart valve microarchitecture when compared with adult
mice. We demonstrate evidence for rather chondrogenic features of valvular interstitial cells by histological staining and
immunodetection of SOX-9, aggrecan, and type 2a1 collagen. Collagen depositions are enriched in a thin layer at the atrial
aspect of atrioventricular valves and the ventricular aspect of bulboventricular valves, respectively. At the ultrastructural
level, the collagen fibrils are lacking obvious periodicity and orientation throughout the entire valve. (J Histochem
Cytochem 67:361–373, 2019)
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:87080 |
| Date | 13 September 2023 |
| Creators | Schulz, Alina, Brendler, Jana, Blaschuk, Orest, Landgraf, Kathrin, Krüger, Martin, Ricken, Albert M. |
| Publisher | Sage Publications |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
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