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Previous issue date: 2012-05-31 / CNPq / The study of autonomic and behavioral responses in dogs subjected to a stress model is crucial at the present time, since animals and humans are constantly subjected to stress in their daily lives. Several stimuli can generate stress in dogs, as areas with crowed of people or animals and sounds of fireworks and thunder. The continuous and repeated exposure to these situations can affect the welfare and health of dogs. The Acupuncture is one of the therapies of Traditional Chinese Medicine (TCM) that targets the prevention and treatment of behavioral and physiological disorders induced by stress, however there are few studies about its efficacy and its mechanisms of action. The present study aimed to evaluate the sympathetic-vagal balance through the analysis of Heart Rate Variability (HRV) and 14 behavioral parameters in response to an acute sound stress in healthy dogs treated with acupuncture. Therefore, we used 24 beagle dogs divided into three groups of 8 animals each: one control group received no treatment (CTL), the second control group received acupuncture treatment at points not corresponding to those used in TCM (NP) and the third group received acupuncture at acupoints VG20, Yintang, PC6, C7 and E36 (ACUP). The treatments lasted 20 minutes and then the animals were subjected to an acute stress model consisting of a sound stimulus (thunders) with intensity of 103-104 dB and duration of 2.5 minutes. The results of HRV analysis showed that during the stress stimulus, acupuncture significantly reduced the LF/HF ratio and the heart rate (HR) compared to NP and CTL. The NP group also had significantly lower LF/HF ratio compared to the CTL during the stress, showing intermediate values between the CTL and ACUP groups. In the behavioral analysis, when compared to CTL group, ACUP showed significantly lower behavioral scores of panting, restlessness, hiding, bolt, startle and escape of the room; and when compared to the NP, ACUP reduced the behavioral scores of hiding, bolt, startle and escape of the room. In addition to reducing significantly the parameters of panting and bolt when compared to the CTL group, the NP group produced intermediate scores between CTL and ACUP groups. The analysis of these data shows that acupuncture treatment promoted reduction of sympatho-vagal balance probably through a sympathetic inhibition associated to parasympathetic stimulation, as well as producing a reduction of behavioral responses of fight and flight characteristics of stress. / O estudo das respostas auton?micas e comportamentais em c?es submetidos a um modelo de estresse se mostra de grande import?ncia na atualidade, uma vez que animais e humanos s?o constantemente submetidos a situa??es de estresse no seu cotidiano. V?rios est?mulos podem ser considerados geradores de estresse como ?reas em que h? aglomera??o de pessoas ou de animais e sons de fogos de artif?cio e de trov?es. A exposi??o continuada e repetida a tais est?mulos pode afetar o bem-estar e a sa?de dos c?es. A acupuntura ? uma das terapias da Medicina Tradicional Chinesa (MTC) que visa o tratamento e a preven??o de dist?rbios fisiol?gicos e comportamentais induzidos pelo estresse, por?m h? poucos estudos na literatura sobre a sua efic?cia e aos seus mecanismos de a??o. O presente estudo teve como objetivo avaliar o balan?o simpato-vagal, atrav?s da an?lise da Variabilidade da Frequ?ncia Card?aca (VFC) e 14 par?metros comportamentais em resposta ao estresse sonoro agudo em c?es saud?veis tratados com acupuntura. Desta forma, foram utilizados 24 c?es da ra?a Beagle divididos em tr?s grupos de 8 animais cada: o primeiro grupo controle n?o recebeu tratamento (CTL), o segundo grupo controle recebeu tratamento por acupuntura em pontos n?o correspondentes aos utilizados na MTC (NP) e o terceiro recebeu acupuntura nos pontos VG20, Yintang, Pc6, C7 e E36 (ACUP). Os tratamentos tiveram dura??o de 20 minutos e em seguida os animais foram submetidos a um modelo de estresse agudo que consistiu de est?mulo sonoro (som de trov?o) com intensidade de 103-104 dB e dura??o de 2,5 minutos. Os resultados da an?lise da VFC demonstraram que durante o est?mulo sonoro a acupuntura significativamente reduziu a raz?o LF/HF e FC m?dia em rela??o aos grupos NP e CTL. O grupo NP tamb?m apresentou valores significativamente menores da raz?o LF/HF em rela??o ao CTL no momento do estresse, apresentando valores intermedi?rios entre os grupos CTL e ACUP. Na an?lise comportamental, a ACUP quando comparado ao grupo CTL apresentou escores comportamentais significativamente menores de arfar, se esconder, inquieta??o, disparada, fugir da sala e sobressalto; e quando comparado ao grupo NP, a ACUP reduziu os escores comportamentais de se esconder, disparada, sobressalto e fugir da sala. Al?m de reduzir significativamente os par?metros de arfar e disparada em rela??o ao CTL, o grupo NP produziu escores intermedi?rios entre CTL e ACUP. A an?lise destes dados demonstra que o tratamento com acupuntura promoveu redu??o do balan?o simpato-vagal provavelmente atrav?s de uma inibi??o simp?tica associada ? estimula??o parassimp?tica, al?m de produzir redu??o das respostas comportamentais de luta e fuga caracter?sticas de estresse.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1506 |
| Date | 31 May 2012 |
| Creators | MACCARIELLO, Carolina Elisabetta Martins |
| Contributors | Medeiros, Magda Alves de, Medeiros, Magda Alves de, Reis, Luis Carlos, Scognamillo, M?rcia Val?ria Rizzo |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Medicina Veterin?ria (Patologia e Ci?ncias Cl?nicas), UFRRJ, Brasil, Instituto de Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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