This thesis is focused on the determination of antioxidants and antioxidant activity. In the first work DMPD method was optimized to determine the antioxidant activity of antioxidants standards and the reaction was monitored depending on the time, temperature and concentration. In the second work automated determination of antioxidant activity of phenolic compounds standards rutin, quercitrine and standards of phenolic acids gallic acid and ferulic acid was tested using five different methods used in the determination of antioxidant activity using an automated pipetting machines and spectrophotometer. In the third work phenolic profile and total antioxidant capacity (TAC) was determined in apricot cultivars with methods high frequency liquid chromatography with tandem electrochemical and UV spectrometric detection and these two methods were compared. In the fourth work electrochemical methods used for the determination of phenolic compounds were described. In the fifth work oxidative stress induced by silver ions in the Staphylococcus aureus bacterial culture was investigated using spectrophotometric and electrochemical methods.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:176879 |
| Date | January 2013 |
| Creators | Dobeš, Jiří |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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