Investigation of Pulse electric field effect on HeLa cells alignment properties on extracellular matrix protein patterned surface

Description

Yes / Cell behavior in terms of adhesion, orientation and guidance, on extracellular matrix (ECM)
molecules including collagen, fibronectin and laminin can be examined using micro contact
printing (MCP). These cell adhesion proteins can direct cellular adhesion, migration,
differentiation and network formation in-vitro. This study investigates the effect of microcontact
printed ECM protein, namely fibronectin, on alignment and morphology of HeLa cells
cultured in-vitro. Fibronectin was stamped on plain glass cover slips to create patterns of
25μm, 50μm and 100μm width. However, HeLa cells seeded on 50μm induced the best
alignment on fibronectin pattern (7.66° ±1.55SD). As a consequence of this, 50μm wide
fibronectin pattern was used to see how fibronectin induced cell guidance of HeLa cells was
influenced by 100μs and single pulse electric fields (PEF) of 1kV/cm. The results indicates that
cells aligned more under pulse electric field exposure (2.33° ±1.52SD) on fibronectin pattern
substrate. Thus, PEF usage on biological cells would appear to enhance cell surface attachment
and cell guidance. Understanding this further may have applications in enhancing tissue graft
generation and potentially wound repair. / Ministry of Higher Education Malaysia and UTHM Tier 1 Research Grant (U865)

Links & Downloads

1. Jamil MMA, Zaltum MAM, Rahman NAA et al (2018) Investigation of Pulse electric field effect on HeLa cells alignment properties on extracellular matrix protein patterned surface. Journal of Physics: Conference Series. 1019(1) 012018.
2. http://hdl.handle.net/10454/16996
3. https://doi.org/10.1088/1742-6596/1019/1/012018

Tags

HeLa

Cells alignment

Pulse electric field

Micro contact printing

Extra cellular matrix

Cell proliferation

Cell guidance

Additional Fields

Identifer	oai:union.ndltd.org:BRADFORD/oai:bradscholars.brad.ac.uk:10454/16996
Date	27 June 2018
Creators	Jamil, M. Mahadi Abdul, Zaltum, M.A.M., Rahman, N.A.A., Ambar, R., Denyer, Morgan C.T., Javed, F., Sefat, Farshid, Mozafari, M., Youseffi, Mansour
Source Sets	Bradford Scholars
Language	English
Detected Language	English
Type	Article, Published version
Rights	Published under licence in Journal of Physics: Conference Series by IOP Publishing Ltd. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence (or successor licence). Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.