Bacterial adhesion is the first step of biofilm formation and in the focus of research interest since several decades. Biofilms cause many problems, sometimes dramatic, for example in health, food packing or waste water purification. Despite of high interest, bacterial adhesion process is only poorly understood yet. In this work, bacterial adhesion was investigated on well-organized and structured model surfaces with various chemistries at molecular scale. For that purpose a characterization methodology was developed to sufficiently analyze monolayers on silicon wafers, and controlled mixed monolayers surfaces with different densities of NH 2 backfilled with CH3 were developed and optimized. These controlled surfaces with different densities of 0 % NH2 up to 100% NH2 were eventually used as tool to study bacterial adhesion in batch and real time conditions. The results demonstrate a significant impact on bacterial adhesion of weak difference in the surface chemistry at molecular scale. In the batch experiments, two so-called "plateaus" zones were determined, in which bacterial adhesion is not significantly different despite the change of the amine concentration on the surface. On the contrary, one transition zone exists between the "plateaus" in which a slight chunge.in the amine concentration leads to a significant increase / decrease of the bacterial adhesion. The same trend of bacteria behavior was observed for different bacterial strains.
| Identifer | oai:union.ndltd.org:CCSD/oai:tel.archives-ouvertes.fr:tel-00847480 |
| Date | 11 September 2012 |
| Creators | Böhmler, Judith |
| Publisher | Université de Haute Alsace - Mulhouse |
| Source Sets | CCSD theses-EN-ligne, France |
| Language | English |
| Detected Language | English |
| Type | PhD thesis |
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