Aspergillus niger DSM 26641 was exposed to 60Co g-radiation to enhance the b-1,4-endoxylanase activity, restrict colony growth and improve robustness of pellets. The first promising mutant obtained after g-radiation of the fungal spores at 50-2000 Gy showed a restricted colony growth and an 82% enhancement in b-1,4-endoxylanase activity. The mutant was subjected to a second round of g-radiation at 1400 Gy generating a mutant with double the b-1,4-endoxylanase activity compared to the native strain. The selected final mutant, deposited as Aspergillus niger DSM 28712, showed a maximal saccharification activity of 26 U·ml-1 on xylan based broth, 48 U·ml-1 on lignocellulose hydrolysate and 375 U·ml-1 on lignocellulose hydrolysate supplemented with yeast extract and mineral salts.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:16854 |
| Date | 01 December 2017 |
| Creators | Ottenheim, Christoph, Werner, Katharina A., Zimmermann, Wolfgang, Wu, Jin Chua |
| Contributors | Universität Leipzig |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/acceptedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 10.1016/j.bej.2014.10.020, 10.1016/j.bej.2014.10.020 |
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