Confocal Raman Microscopy, a non-invasive, non-destructive and label-free
technique, was employed to study the uptake and localization of nanoparticles (NPs) in the
Hepatocarcinoma human cell line HepG2 at the level of single cells. Cells were exposed to
carbon nanotubes (CNTs) the surface of which was engineered with polyelectrolytes and lipid
layers, aluminium oxide and cerium dioxide nanoparticles. Raman spectra deconvolution was
applied to obtain the spatial distributions of NPs together with lipids/proteins in cells. The
colocalization of the NPs with different intracellular environments, lipid bodies, protein and
DNA, was inferred. Lipid coated CNTs associated preferentially with lipid rich regions,
whereas polyelectrolyte coated CNTs were excluded from lipid rich regions. Al2O3 NPs were
found in the cytoplasm. CeO2 NPs were readily taken up and have been observed all over the
cell. Raman z-scans proved the intracellular distribution of the respective NPs.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:80180 |
| Date | 27 July 2022 |
| Creators | Estrela-Lopis, Irina, Romero, G., Rojas, E., Moya, Sergio E., Donath, Edwin |
| Publisher | IOP Publishing |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 012017 |
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