The surfactant protein-G (SP-G) has recently been discovered in the brain and linked to
fluid balance regulations. Stroke is characterized by impaired vessel integrity, promoting water
influx and edema formation. The neurovascular unit concept (NVU) has been generated to cover not
only ischemic affections of neurons or vessels but also other regionally associated cells. This study
provides the first spatio-temporal characterization of SP-G and NVU elements after experimental
stroke. Immunofluorescence labeling was applied to explore SP-G, vascular and cellular markers
in mice (4, 24, and 72 h of ischemia), rats (24 h of ischemia), and sheep (two weeks of ischemia).
Extravasated albumin indicated vascular damage within ischemic areas. Quantifications revealed
decreasing SP-G signals in the ischemia-affected neocortex and subcortex. Inverse immunosignals
of SP-G and vascular elements existed throughout all models. Despite local associations between
SP-G and the vasculature, a definite co-localization was not seen. Along with a decreased SP-
G-immunoreactivity in ischemic areas, signals originating from neurons, glial elements, and the
extracellular matrix exhibited morphological alterations or changed intensities. Collectively, this
study revealed regional alterations of SP-G, vascular, and non-vascular NVU elements after ischemia,
and may thus stimulate the discussion about the role of SP-G during stroke.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:89161 |
| Date | 20 January 2024 |
| Creators | Michalski, Dominik, Reimann, Willi, Spielvogel, Emma, Mages, Bianca, Biedermann, Bernd, Barthel, Henryk, Nitzsche, Björn, Schob, Stefan, Härtig, Wolfgang |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 5875 |
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