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Chemical Cross-Linking and Its Effect on Fatty Acid Synthetase Activity in Intact Chloroplasts From Euglena gracilis

Intact chloroplasts were isolated from Euglena gracilis variety bacillaris, and aliquots were exposed to several different chemical cross-linking reagents. The reagents penetrated the triple membrane of Euglena chloroplasts. This was shown by gradient acrylamide gel electrophoresis under denaturing conditions. The activity of the nonaggregated fatty acid synthetase of Euglena was located within the chloroplast stroma, and the effects of dimethylsuberimidate cross-linking on the activity of the enzyme system were examined. The acyl-carrier protein concentration in the chloroplast was measured at about 0.24 mM.

Identiferoai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etsu-works-12727
Date16 December 1988
CreatorsWorsham, Lesa M., Tucker, Margie M., Lou Ernst-Fonberg, Mary
PublisherDigital Commons @ East Tennessee State University
Source SetsEast Tennessee State University
Detected LanguageEnglish
Typetext
SourceETSU Faculty Works

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