Orientador : Prof. Dr. Luciano dos Santos Bersot / Dissertação (mestrado) - Universidade Federal do Paraná, Setor de Palotina, Programa de Pós-Graduação em Ciência Animal. Defesa: Palotina, 23/02/2017 / Inclui referências : f. 83-102 / Linha de pesquisa: Microbiologia / Resumo: O objetivo deste trabalho foi verificar a ocorrência, quantificar e identificar os sorotipos de Salmonella sp. presentes em cortes de frango congelados produzidos e comercializados em todo o Estado do Paraná. Foram avaliados 300 cortes de frango variados, coletados no varejo, em todas as regiões produtoras do estado do Paraná. Todas as amostras passaram por uma avaliação inicial de sua embalagem, onde foi verificada a integridade e o atendimento às normas legais. A quantificação de Salmonella sp. foi obtida por meio da metodologia de mNMP, determinada pela ISO/TS 6579-2:2012. Para verificação da presença/ausência utilizou-se a metodologia ISO/TS 6579:2002. Também foi realizada a quantificação de coliformes termotolerantes em 154 dessas amostras, avaliando sua conformidade em relação à RDC nº 12 de 2001. Além disso, foram realizados testes fenotípicos e genotípicos de suscetibilidade antimicrobiana e produção de enzimas ESBL. Das 300 amostras analisadas, 95 (31,5%) apresentaram Salmonella sp., sendo os sorotipos identificados como Typhimurium (43%), Heidelberg (39%), Ndolo (6%), Minnesota (4%), O:4,5 (2%), Thompson (2%), Schwarzengrund (2%), O:3, 10:e, h:- (1%) e Abony (1%). A quantificação demonstrou baixa carga de Salmonella sp., variando de 0,12 a 6,4 NMP/g. Todas a 154 amostras apresentam contagem de coliformes termotolerantes de acordo com os padrões legais. Foi contatada a presença de perfil multirresistente em 85 (86,7%) isolados de Salmonella sp., sendo que 13 desses demonstraram possuir genes que codificam enzimas ESBL, especialmente blaCTX-M-2 e blaTEM-1. Palavras-chave: blaCTX-M-2. Dose Infectante. Multirresistência. S. Heidelberg. S. Ndolo. S. Typhimurium. / Abstract: The objective of this work was to verify the occurrence, quantify and identify the serotypes of Salmonella sp. present in frozen chicken parts produced and marketed throughout the State of Paraná. A total of 300 varied chicken parts were collected in the retail market in all producing regions of the state of Paraná. All the samples underwent an initial assessment of their packaging, where the integrity and compliance with legal regulations were verified. The quantification of Salmonella sp. was obtained using the methodology of mNMP, determined by ISO/ST 6579-2:2012. ISO/ST 6579:2002 methodology was used to verify presence/absence. Quantification of thermotolerant coliforms was also carried out in 154 of these samples, evaluating their compliance with RDC nº 12 of 2001. In addition, phenotypic and genotypic tests of antimicrobial susceptibility and ESBL production were performed. Of the 300 samples analyzed, 95 (31.5%) presented Salmonella sp., with serotypes identified as Typhimurium (43%), Heidelberg (39%), Ndolo (6%), Minnesota (4%), O: 4.5 (2%), Thompson (2%), Schwarzengrund (2%), 0: 3, 10: e, h :- (1%) and Abony (1%). Quantification showed low Salmonella sp. loading, ranging from 0.12 to 6.4 MPN/g. All the 154 samples present count of thermotolerant coliforms according to the legal standards. The presence of a multidrug-resistant profile in 85 (86.7%) of Salmonella sp. strains was reported, 13 of which were found to have ESBL-encoding genes, especially blaCTX-M-2 and blaTEM-1. Key-words: blaCTX-M-2. Infectant dose. Multiresistance. S. Heidelberg. S. Ndolo. S. Typhimurium. / Abstract: The objective of this work was to verify the occurrence, quantify and identify the serotypes of Salmonella sp. present in frozen chicken parts produced and marketed throughout the State of Paraná. A total of 300 varied chicken parts were collected in the retail market in all producing regions of the state of Paraná. All the samples underwent an initial assessment of their packaging, where the integrity and compliance with legal regulations were verified. The quantification of Salmonella sp. was obtained using the methodology of mNMP, determined by ISO/ST 6579-2:2012. ISO/ST 6579:2002 methodology was used to verify presence/absence. Quantification of thermotolerant coliforms was also carried out in 154 of these samples, evaluating their compliance with RDC nº 12 of 2001. In addition, phenotypic and genotypic tests of antimicrobial susceptibility and ESBL production were performed. Of the 300 samples analyzed, 95 (31.5%) presented Salmonella sp., with serotypes identified as Typhimurium (43%), Heidelberg (39%), Ndolo (6%), Minnesota (4%), O: 4.5 (2%), Thompson (2%), Schwarzengrund (2%), 0: 3, 10: e, h :- (1%) and Abony (1%). Quantification showed low Salmonella sp. loading, ranging from 0.12 to 6.4 MPN/g. All the 154 samples present count of thermotolerant coliforms according to the legal standards. The presence of a multidrug-resistant profile in 85 (86.7%) of Salmonella sp. strains was reported, 13 of which were found to have ESBL-encoding genes, especially blaCTX-M-2 and blaTEM-1. Key-words: blaCTX-M-2. Infectant dose. Multiresistance. S. Heidelberg. S. Ndolo. S. Typhimurium.
| Identifer | oai:union.ndltd.org:IBICT/oai:dspace.c3sl.ufpr.br:1884/46125 |
| Date | 15 May 2017 |
| Creators | Perin, Ana Paula |
| Contributors | Universidade Federal do Paraná. Setor Palotina. Programa de Pós-Graduação em Ciência Animal, Bersot, Luciano dos Santos |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | 103f. : il., algumas color., tabs., grafs., maps., application/pdf |
| Source | reponame:Repositório Institucional da UFPR, instname:Universidade Federal do Paraná, instacron:UFPR |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | Disponível em formato digital |
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