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Previous issue date: 2010-03-05 / Planos de Reestrutura??o e Expans?o das Universidades Federais (Reuni) / Even with technological advances in the field of artificial insemination, there is a huge
number of people freezing semen in a manual way. This technique has proven to be viable,
however, it is difficult to standardize the cooling and freezing curves, since they depend on
the quality of the material used (refrigerator sealing, type of cooler, liquid nitrogen level and
other). Therefore, the objective of this study was to evaluate the effect of the cryopreservation
of bovine semen using two freezing techniques (conventional and automated) on motility,
vigor and heat resistance of the sperm after thawing. The experiment was developed over a
period of 12 weeks, divided into two parts, an initial six weeks part where Tris-yolk extender
was used and a second part in which the extender used was Citrate-yolk. Three Holstein bulls
were used for weekly semen collection by artificial vagina method. After collection, were
evaluated: volume, motility, mass motility and sperm concentration to find out the number of
doses. After dilution, the semen was stored in straws of 0.5 ml. Then the straws were
subjected to conventional freezing technique (use of refrigerator, nitrogen vapor and
immersion in liquid nitrogen) and automated technique (machine from Cryogen ?). After 24
hours the samples were thawed, re-tested and underwent the quick thermoresistance test. The
results were analyzed by Kruskal-Wallis analysis of variance (nonparametric) with a
significance level of 5% with the aid of BioEstat 4 and Systat 11 PC-programs. There was
significant difference (p <0.05) between automated and manual techniques for both extenders
used, and the automated technique has presented the best results for motility and vigor after
cryopreservation and thermoresistance test. Thus, we can conclude that the automated
technique to freezing bovine semen has shown to be advantageous and practical because as
well as providing better results, it standardizes cooling and freezing curves. / Mesmo com os avan?os tecnol?gicos no campo da insemina??o artificial, ainda ? grande o
n?mero de pessoas que realizam a congela??o de s?men de maneira manual. Esta t?cnica tem
se mostrado vi?vel, por?m, ? dif?cil padronizar as curvas de resfriamento e de congela??o,
uma vez que dependem da qualidade do material utilizado (veda??o da geladeira, tipo de
caixa de isopor, quantidade de gelo, n?vel de nitrog?nio l?quido e outros). Portanto, o objetivo
deste estudo foi avaliar o efeito da criopreserva??o de s?men bovino utilizando duas t?cnicas
de congela??o (convencional e automatizada) sobre a motilidade, vigor e resist?ncia t?rmica
dos espermatoz?ides ap?s a descongela??o. O experimento foi elaborado em um per?odo de
12 semanas, sendo dividido em duas partes, uma inicial de seis semanas onde foi utilizado o
diluidor Tris-gema e uma segunda parte na qual o diluidor utilizado foi Citrato-gema. Foram
utilizados tr?s touros holandeses para coleta de s?men semanal pelo m?todo da vagina
artificial. Ap?s a coleta, foram avaliados: volume, motilidade, turbilhonamento, vigor e
concentra??o esperm?tica para determina??o do n?mero de doses. Ap?s dilui??o, o s?men foi
envasado em palhetas de 0,5 ml. Em seguida, as palhetas foram submetidas ?s t?cnicas de
congela??o convencional (uso de geladeira, vapor de nitrog?nio e imers?o em nitrog?nio
l?quido) e automatizada (aparelho da Cryogen?). Ap?s 24 horas as amostras foram
descongeladas, reavaliadas e submetidas ao teste de termoresist?ncia r?pido (TTR). Os
resultados foram analisados pelo teste Kruskal-Wallis (an?lise de vari?ncia n?o param?trica)
com n?vel de signific?ncia de 5% com aux?lio dos programas BioEstat 4 e Systat 11. Houve
diferen?a significativa (p<0,05) entre as t?cnicas automatizada e manual para os dois
diluidores testados, sendo a t?cnica automatizada a que apresentou melhores resultados para
motilidade e vigor ap?s a criopreserva??o e teste de resist?ncia t?rmica. Desta forma, pode-se
concluir que a t?cnica automatizada para congela??o de s?men bovino apresenta-se vantajosa
e pr?tica pois al?m de proporcionar melhores resultados, ela padroniza as curvas de
refrigera??o e de congelamento.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1903 |
| Date | 05 March 2010 |
| Creators | Vasconcelos Filho, Wilson Franklim de |
| Contributors | Mello, Marco Roberto Bourg de, Dias, Angelo Jos? Burla, Jesus, Vera L?cia Teixeira de |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Zootecnia, UFRRJ, Brasil, Instituto de Zootecnia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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