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Previous issue date: 2017-02-20 / CAPES / Consumers are increasingly becoming more critical in the selection of a food product, by mainly considering sensory, nutritional and safety issues in the decision to purchase. As a result, less drastic preservation methods have been more deeply investigated and broadly adopted in industrial scale, including the addition of natural antimicrobials and the high hydrostatic pressure processing. In this sense, the objective of the this study was to evaluate the effect of the incorporation of carrot either individually or in combination with high pressure tretament on the inhibition of Listeria innocua inoculated in Minas Frescal cheese, besides evaluating the effect on the proximate composition, carotenoids content and sensorial characteristics and acceptance. A 22 factorial design was first employed to perform the chemical and physical characterization, being the independent variables the pressure (0 and 500 MPa) and the carrot concentration (0% and 6%), with three repetitions of the central point. The following analyses were carried out in the cheeses resulting from the treatments: proximate composition (moisture, protein, ethereal extract, fixed mineral residue - FMR); instrumental color; carotenoid quantification (total carotenoids, lutein, ?-carotene and ?-carotene) and enumeration of Listeria innocua. For the sensory analysis, a full factorial design was adopted, by considering the central points of the first design as factors themselves. The global acceptance was evaluated by a 9-point hedonic scale and the CATA Test (check-all-that-apply) with 102 consumers. High pressure processing at the level of 500 MPa for 10 minutes reduced the Listeria innocua count from 7 log cfu /ml up to undetectable levels, without further sigificant increase along the chilled storage at 8 ?C for 22 days. On the contrary, the single carrot incorporation (6%) or its combination in the level of (3%) with pressure treatment at (250 MPa) had no effect on the inhibition of this microorganism. Moisture content, protein, ethereal extract and FMR were not significantly affected by either the carrot incorporation or the high pressure processing. As expected, carrot addition enhanced the levels of total carotenoids, lutein, ?-carotene and ?-carotene, as well as affected the instrumental color parameters. There again, high pressure processing had no significant effect on these compounds. Furthermore, high hydrostatic pressure processing did not influence the overall acceptance of the samples, while the incorporation of 6% of carrot tended to lower the acceptance. In the analyzis of individual consumers, the cheese with 3% of carrot processed at 250 MPa did not differ from the control sample, which was the most preferred sample. According to the CATA test, the consumers were able to sensory characterize and differentiate between the cheeses with or without the addition of carrot. / Os consumidores t?m se tornado mais cr?ticos na escolha de um produto aliment?cio, sendo as caracter?sticas sensoriais, nutricionais e funcionais, assim como a seguran?a do alimento, fatores preponderantes na decis?o de compra do consumidor. Tais demandas t?m induzido a busca por m?todos alternativos de conserva??o, menos danosos ? qualidade do produto, como adi??o de antimicrobianos naturais e o processamento por alta press?o hidrost?tica. Nesse sentido, o presente estudo objetivou avaliar o efeito da incorpora??o da cenoura e do tratamento por alta press?o hidrost?tica na inibi??o da Listeria innocua e nas caracter?sticas qu?micas, f?sica, microbiol?gicas e sensoriais do queijo Minas Frescal, visando desenvolver um produto diferenciado, mais seguro e saud?vel. O processamento dos queijos seguiu um delineamento fatorial 22 com os fatores: press?o (0 e 500 MPa) e concentra??o de cenoura (0 e 6%) com 3 repeti??es do ponto central. Foram realizadas an?lises f?sico-quimicas (umidade, prote?na, extrato et?reo, res?duo mineral fixo (RMF) e cor instrumental), quantifica??o dos carotenoides (carotenoides totais, lute?na, ?-caroteno e ?-caroteno) e contagem de Listeria innocua. A an?lise sensorial seguiu um delineamento fatorial completo (considerando os pontos centrais como fatores), em que foi avaliado a aceita??o global pela escala hed?nica de 9 pontos e o Teste CATA (check-all-that-apply) com 102 consumidores. O processamento por alta press?o em 500 MPa por 10 minutos reduziu a contagem de Listeria innocua em aproximadamente 7 log ufc/mL, para n?veis n?o detect?veis imediatamente ap?s o processamento, e essa contagem se manteve constante ao longo do armazenamento a 8?C por 22 dias. J? a incorpora??o individual (sem pressuriza??o) da cenoura (6%), ou a utiliza??o combinada desta na concentra??o de 3% com a press?o de 250 MPa, n?o apresentou efeito na inibi??o desse micro-organismo. Os teores de umidade, prote?na, extrato et?reo e RMF tamb?m n?o foram afetados significativamente pela incorpora??o da cenoura ou pelo processamento de alta press?o hidrost?tica. Como esperado, a incorpora??o da cenoura aumentou os teores de carotenoides totais, lute?na, ?-caroteno e ?-caroteno, bem como influenciou nos par?metros de cor instrumental. Em contrapartida, o processamento por alta press?o n?o apresentou efeito significativo sobre esses compostos. O processamento por alta press?o hidrost?tica pouco influenciou na aceita??o global da amostras, enquanto que a incorpora??o de 6% de cenoura tendeu a uma menor aceita??o. Quando analisados os consumidores individualmente, foi verificado que a amostra processada a 250 MPa com 3% de cenoura n?o diferiu da amostra controle, sendo essas as mais preferidas. O teste CATA mostrou que os consumidores conseguiram diferenciar e caracterizar sensorialmente os queijos incorporados com cenoura dos n?o adicionados.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/2006 |
| Date | 20 February 2017 |
| Creators | GOUVEA, Fabiola dos Santos |
| Contributors | Rosenthal, Amauri, Ferreira, Elisa Helena da Rocha, Rosenthal, Amauri, Tiburski, Julia Hauck, Paula, Junio Cesar Jacinto de |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos, UFRRJ, Brasil, Instituto de Tecnologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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