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Previous issue date: 2010-08-19 / Regarding the measurement of tear production, the technique of reading tear with a cotton soaked in a pH indicator solution has proven to be advantageous compared to Schirmer Tears Test in many studies due to the smaller width of the test material and time for attaining this , which leads to less reflex tear production and a closer reading of basal tear production or real. This experiment aimed to develop a quantitative measurement equipment in tear production similar to the ZoneQuick? Menicon and improve it, establish a pattern of reading to those and evaluate their advantages and disadvantages. For that, we selected 50 beagle dogs, male and female, aged 1-8 years from the Canil do Laboratorio de Quimioterapia Experimental Parasitol?gica of UFRRJ, and produced two tests with Cotton Thread with Phenol Red and Bromocresol Green. Afterwards was made the reading standization of those three tests compared to the Schirmer tear test, getting as normal range of reading for the tests with Cotton Thread with Phenol Red 13.6 to 22.7 mm/15seg., the ZoneQuick? Menicon of between 22, 7 and 37.8 mm/15seg. and tests with Cotton Thread with the Bromocresol Green 13.7 to 22.8 mm/15seg.. Among those three tests, the ZoneQuick? showed the smallest shift in color of the indicator and the largest Cotton Thread with the Bromocresol Green which along with the smaller standard error presented by this facilitates its reading and reduces the chance of false positive or negative. In a comparison between the Schirmer Tears Test , Cotton Thread with the Bromocresol Green tests and Cotton Thread with Phenol Red latter, generated less reflex tear production, with a reading closer to the real. / Em rela??o ? mensura??o da produ??o lacrimal, a t?cnica de leitura lacrimal com o fio de algod?o embebido em uma solu??o indicadora de pH vem se mostrando vantajosa em rela??o ao Teste Lacrimal de Schirmer I (TLS I) em diversos estudos, devido ? menor largura do material de teste e tempo de realiza??o deste, o que leva a uma menor produ??o lacrimal reflexa e uma leitura mais pr?xima da produ??o lacrimal basal ou real. Este experimento teve como objetivos, testar diferentes tipos de fios e avaliar qual o mais indicado a realiza??o dos testes, desenvolver um material de medi??o quantitativo da produ??o lacrimal de fio impregnado com vermelho fenol, testar outro indicador de pH, estabelecer um padr?o de leitura para estes e o ZoneQuick? da Menicon e compar?-los ao TLS I avaliando suas vantagens e desvantagens. Para isto, foram selecionados 50 c?es da ra?a Beagle, machos e f?meas, com idade variando de 1 a 8 anos do Canil do Laborat?rio de Quimioterapia Experimental Parasitol?gica da UFRRJ, e produzidos dois testes com Fio de Algod?o impregnados com Vermelho Fenol ou Verde de Bromocresol. Em seguida foi feita a padroniza??o da leitura dos tr?s testes em compara??o ao TLS I, obtendo-se como faixa normal de leitura para o Teste de Fio de Algod?o com Vermelho Fenol de 13,6 a 22,7 mm/15seg., o ZoneQuick? da Menicon entre 22,7 e 37,8mm/15seg. e o Teste de Fio de Algod?o com Verde de Bromocresol de 13,7 a 22,8 mm/15 seg.. Dentre os tr?s testes, o ZoneQuick? apresentou a menor viragem na cor do indicador e o Teste de Fio de Algod?o com Verde de Bromocresol a maior o que, juntamente com o menor erro padr?o por este apresentado, facilita sua leitura e reduz a chance de falso positivo ou negativo. Na etapa de compara??o entre os TLS I, Fio de Algod?o com Verde de Bromocresol e Fio de Algod?o com Vermelho Fenol este ?ltimo gerou menor produ??o lacrimal reflexa, com uma leitura mais pr?xima da real.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/2455 |
| Date | 19 August 2010 |
| Creators | Silva, Leandro Nogueira e |
| Contributors | Pereira, Jo?o Telhado |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Medicina Veterin?ria (Patologia e Ci?ncias Cl?nicas), UFRRJ, Brasil, Instituto de Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | ADAMS A. D., Conjunctival surface mucus. In. Holly F. J., The Preocular Tear Film in Health, Disease, and Contact Lens Wear, Dry Eye Institute, Inc, Lubbock, Texas, p. 304?311, 1986. AGUIRRE G.; RUBIN L.F.; BISTNER S. I., The development of the canine eye. American journal of veterinary research v. 33, p. 2399?2414, dez.1972. AGUIRRE G. D., RUBIN L. F., HARVEY C. E. Keratoconjunctivitis sicca in dogs. Journal of the American Veterinary Medical Association, v. 158, p. 1566?1579, Mai. 1971. ALMEIDA D. E., ROVERATTI C., BRITO F. L. C., GODOY G. S., DUQUE J. C. M., BECHARA G. H. and LAUS J. L.; Conjunctival effects of canine distemper virus-induced keratoconjunctivitis sicca, Blackwell Publishing, Ltd. Veterinary Ophthalmology, v. 12, issue 4, p. 211?215, 2009. AMIN A. S., Quantitative determination of some pharmaceutical veterinary formulations using bromocresol purple and bromocresol green. Analiticals Letters, vol 30, n?14, p. 2503-2513 ASHUTOSH S. Energetics of corneal epithelial cell-ocular mucus?tear film interactions: some surface-chemical pathways of corneal defense. Biophysical Chemistry, v. 47, p. 87?99, 1993. BARABINO S., CHEN W., DANA M. R., Tear film and ocular surface tests in animal models of dry eye: uses and limitations, Experimental Eye Research, v. 79, p. 613?621, 2004. BARNETT K.C., CRISPIN S.M., Lacrimal System, Feline ophthalmology, WB Saunders, London, p. 61?68, 1998 BERGER S.L.; KING V.L. The fluctuation of tear production in the dog. Journal American Animal Hospital Association, v. 34, p. 79?83, jan/fev 1998. BROWN M.H. The phenol red tear test in healthy cats. Veterinary and Comparative Ophthalmology, v. 7, p. 249-252, 1997. CARRINGTON S.D. Polarized light biomicroscopic observations on the pre-corneal tear film.1 . 35 The normal tear film of the dog. Journal Small Animal Practice, v. 28, p. 605?622, 1987. CHANDLER J. W., GILLETTE T. E., Immunologic defense mechanisms of the ocular surface. Ophthalmology, v. 90, p. 585?591, 1983. CLERC B. Epiphora et larmoiement ou ? l?oeil qui pleure ?. Point V?terinaire, v. 18, p. 255?261 and 369-380, 1986. CORFIELD A. P., CARRINGTON S. D., HICKIS S. J. et al. Ocular mucins: purification, metabolism, and functions. Progress in Retinal and Eye Research, v. 16, p. 627?656, 1997. CULLEN C. L., NJAA B. L. and GRAHN B. H., Ulcerative Keratitis associated with qualitative tears film abnormalities in cats , Blackwell Publishing Inc., Veterinary Ophthalmology, v. 2, issue 3, p. 197 ? 204, Sep. 1999. CULLEN C. L., IHLE S. L., WEBB A. A. and MC CARVILLE C., Keratoconjunctival effects of diabetes mellitus in dogs, Blackwell Publishing, Ltd., Veterinary Ophthalmology, v. 8, issue 4, p. 215?224, Jul-Ago 2005. DARTT D. A., Regulation of tear secretion. Advances in Experimental Medicine and Biology, v 350, p. 1-10, 1994. DARTT D. A., MCCARTHY D. M., MERCER H. J., KESSLER T. L., CHUNG E. H., ZIESKE J. D. Localization of nerves adjacent to goblet cells in rat conjunctiva. Current Eye Research, v. 14, p. 993?1000, 1995. DAVIDSON H. J., KUONEN V. J., The tear film and ocular mucins, Veterinary Ophthalmology Blackwell Publishing Ltd., p. 7, issue 2, p. 71?77, 2004. DEAN E. Techniques d?examen de l?oeil. In. Ophtalmologie Du Chien, Pratique M?dicale & Chirurgicale d l?animal de compagnie; v. 32, p. 21?28, 1997. DILLY P. N., Conjunctival cells, subsurface vesicles, and tear film mucus. In. Holly F. J., The Preocular Tear Film in Health, Disease, and Contact Lens Wear, Dry Eye Institute, Inc, Lubbock, Texas, p. 677?687, 1986 36 DRIVER P. J., LEMP M. A., Meibomian gland dysfunction. Survey of Ophthalmology, v. 40, p. 343?363, 1996. EVANS H.E.; Christensen G.C. Miller?s anatomy of the dog. 2? ed. Philadelphia: WB Saunders; p. 256?259, 1979. GELATT K.N.; PEIFFER Jr. R.L.; ERICKSON J.L.; GUM G.G. Evaluation of tear formation in the dog using a modification of Schirmer tear test. Journal American Veterinary Medical Association, v. 166, p. 368?370, 1975. GERMAN A. J., HALL E. J., DAY M. J. Measurement of IgG, IgM and IgA concentrations in canine serum, saliva, tears and bile. Veterinary Immunology and Immunopathology, v. 64, p. 107?121, 1998. GRAHN B. H., STOREY E. S., Lacrimostimulants and lacrimomimetics, Veterinary Clinical Small Animal, 34 (2004) 739?753 GUM G..G..; GELATT K. N.; OFRI R., Physiology of the eye. In: GELATT K. N., editor. Veterinary ophthalmology. Philadelphia: LEA and FEBIGER; p. 124?161, 1999. HAKANSON N.W.; ARNESSON K. Temporal variation in tear production in normal beagle dogs as determined by Schirmer tear test. Veterinary and Comparative Ophthalmology v. 7, p. 196?203, 1997. HARTLEY C., WILLINMS D. L., ADAMS V. J., Effect of age, gender, weight, and time of day on tear production in normal dogs, Veterinary Ophthalmology, v. 9, p. 53?57, Jan-Feb 2006. HELPER L. The effect of lacrimal gland removal of the conjunctiva and cornea of the dog. American Journal Veterinary Medical Association. v. 157, p. 72?75, 1970. HIDA R. Y., NISHIWAKI-DANTAS M. C., HIDA M. M., TSUBOTA K. Quantitative tears study using the red phenol test in the Brazilian population. Departamento de oftalmologia ? Faculdade de Ci?ncias M?dicas da Santa Casa de Misericordia de S?o Paulo, Arquivos Brasileiros de Oftalmologia, v. 68, n?.4, July./Aug. 2005. 37 HOLT E., ROSENTHAL K., and SHOFER F. S. The phenol red thread tear test in large Psittaciformes, Veterinary Ophthalmology, v. 9, n? 2, p. 109?113, 2006. IWATA S. Chemical composition of the aqueous phase. International Ophthalmology Clinics, v. 13, p. 29-46, 1983. KASWAN R.L., MARTIN C.L., DAWE D.L., Keratoconjunctivitis sicca: immunological evaluation of 62 canine cases. American Journal of Veterinary Research, v. 46, p. 376-383, 1985. MARTIN C. L., ANDERSON C. B. Ocular anatomy. In: GELATT K. N., editor. Textbook of veterinary ophthalmology. Philadelphia: LEA and FEBIGER; 1981. p. 12?121. MCKENZIE R. W., JUMBLATT J. E., JUMBLATT M. M., Quantification of MUC2 and MUC5AC transcripts in human conjunctiva. Investigative Ophthalmology and Visual Science, v. 41, p. 703?708, 2000. MCLAUGHLIN S. A., BRIGHTMAN 2nd A. H., HELPER L. C., PRIMM N. D., BROWN M. G., GREELEY S. Effect of removal of lacrimal and third eyelid glands on Schirmer tear test results in cats. Journal American Veterinary Medical Association, v. 193, p. 820?822, 1988. MOORE C. P. Qualitative tear film disease in small animal ophthalmology. Veterinary Clinical North American Small Animal, v. 20, p. 565?581,1990. MOORE C. P. Diseases and Surgery of the Lacrimal Secretory System, . In: GELATT K. N., editor. Veterinary ophthalmology. Philadelphia: LEA and FEBIGER; p. 583-608, 1999. NICHOLS B.A., CHIAPPINO M.L., DAWSON C.R. Demonstration of the mucous layer of the tear film by electron microscopy. Investigative Ophthalmology and Visual Science, v. 26, p. 464-473, 1985. OFRI R., ORGAD K., Philip H. KASS P. H., DIKSTEIN S., Canine meibometry: Establishing baseline values for meibomian gland secretions in dogs, The Veterinary Journal, v. 174, p. 536?540, 2007. 38 PICCIONE G. GIANNETTO, C., FAZIO F., ASSENZA A., CAOLA G. Daily rhythm of tear production in normal dog maintained under different Light/Dark cycles Research in Veterinary Science, v. 86, p. 521?524, 2009. POWELL C. C, MARTIN C. L. Distribution of cholinergic and adrenergic nerve fibers in the lacrimal glands of dogs. American Journal of Veterinary Research, v. 50, p. 2084?2088, 1989. PRINCE J. H., DIESEN C. D., EGLITIS I., RUSKELL G. L., Anatomy and histology of the eyes and orbit in domestic animals. Springfield: CC Thomas; p. 220?225, 1960. RIOS J. D., FORDE K., DIEBOLD Y. et al. Development of conjunctival goblet cells and their neuroreceptor subtype expression. Investigative Ophthalmology and Visual Science, v. 41, p. 2127?2137, 2000. ROLANDO M. D., ZIERHUT M. D., The Ocular Surface and Tear Film and Thei Dysfunction in Dry Eye Disease, Survey of Ophthalmology, v. 45, issue 2, p. S203-S209, Mar. 2001. SAITO A., IZUMISAWA Y., YAMASHITA K., KOTANI T. The effect of third eyelid gland removal on the ocular surface of dogs. Veterinary Ophthalmology, v. 4, p. 13?18, mar. 2001. SAITO A, KOTANI T., Stimation of lacrimal level and testing methods on normal Beagle, Veterinary Ophthalmology, v. 4, p. 7-11, 2001. SAMUELSON D. A. Ophthalmic Anatomy, In: GELATT K. N., editor. Veterinary ophthalmology. Philadelphia: LEA and FEBIGER; p. 31-150, 1999 SANSOM J., BARNETT K. C. Keratoconjunctivitis sicca in the dog: a review of two hundred cases. Journal of Small Animal Practice, v. 26, p. 121?131, 1985. SCOTTI S.,KLEIN A., ANORE M. V , HIDALGO A., AYOLLE P. F AND MOISSONNIER P., A new surgical method for the control of the epiphora in dogs: modified parotid duct transposition, Journal of Small Animal Practice, v. 48, issue 5, p. 279?282, May. 2007. 39 SLATTER D., Lacrimal System. Fundamentals of Ophthalmology Veterinary 3rd. W. B. Saunders Company, 2005, p.259-282 STILES J, MCDERMOTT M, BIGSBY D, WILLIS M, MATIN C, ROBERTS W, GREENE C. Use of nested polymerase chain reaction to identify feline herpes virus in ocular tissues from clinically normal cats and cats with corneal sequestra or conjunctivitis. American Journal of Veterinary Research, v. 58, p. 338?342, Apr. 1997. STRUBBE D. T., GELATT K. N., Ophthalmic Examination and Diagnostic Procedures, In: GELATT K. N., editor. Veterinary ophthalmology. Philadelphia: LEA and FEBIGER; p. 427-466, 1999. TERCI D. B. L. AND ROSSI A. V., Indicadores naturais de pH: usar papel ou solu??o?. Qu?mica Nova. Vol. 25, n? 4, 684-688 TROST K., SKALICKY M. AND NELL B., Schirmer tear test, phenol red thread tear test, eye blink frequency and corneal sensitivity in the guinea pig, Veterinary Ophthalmology, v. 10, n. 3, p. 143-146, 2007. WESTERMEYER H. D., WARD D. A. and ABRAMS K., Breed predisposition to congenital alacrima in dogs, Blackwell Publishing Inc., Veterinary Ophthalmology, v. 12, issue 1, p. 1?5 Jan-Feb. 2009. WILLIAMS D. L., Analysis of tear uptake by the Schirmer tear test strip in the canine eye, Blackwell Publishing, Ltd., Veterinary Ophthalmology, v. 8, n? 5, p. 325?330, Sep. 2005. WILLIAMS D. L, PIERCE V., ELLOR P. M. and HEATH M. F., Reduced tear production in three canine endocrinopathies, Journal of Small Animal Practice, v. 48, issue 5, p. 252?256, May. 2007. |
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