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Previous issue date: 2008-02-25 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The objective of the present study was to establish trial infections in adult dogs by
administering T. canis larvae recovered from tissues of experimentally infected mice and to
evaluate patent infection by determining the pre-patent period (PPP) and the number of
recovered nematodes in relation to the different infective larvae doses. T. canis females were
collected from naturally infected dogs. These were dissected and the eggs were obtained from
their uteri followed by posterior incubation at 27?1? C under 75 ?10% RH. After 60 days,
mice were orally infected by the embryonated eggs gathered from the culture. The mice were
killed 26 days post-infection (dpi) and then submitted to necropsy. Its organs and carcasses
were processed by the Acid-Isolation Technique for the recovery of larvae, which were
quantified and subdivided in aliquots for the dogs infection. Twelve adult male Beagle dogs
from the Kennel of the Laboratory of Experimental Chemotherapy in Veterinary Parasitology,
Department of Animal Parasitology, Veterinary Institute, Universidade Federal Rural do Rio
de Janeiro were used in our study. They were considered free from parasitism, however with
previous history of exposition. The animals were divided in two groups of six animals each.
The dogs from Group I were infected with 60 larvae, and the ones from Group II with 300
larvae, which were recovered from the brains and carcasses of the paratenic hosts. To evaluate
the PPP we used the coproparasitological techniques of Centrifugal flotation, Sedimentation
and Sedimentation centrifugal flotation on days +8, + 12 and, consecutively between days
+16 e + 56. For the recovery of nematodes from feces, the dogs were treated on days +57, +58
e +59 with piperazine. The animals from Group I reached the PPP on 48?5.37 dpi, and Group
II on 41.5?5.09 dpi, presenting statistically significant difference between the periods
(p<0.05). Ten nematodes were collected from Group I, with the mean intensity of 1.66?1.63,
resulting in 2.78?2.73% of recovery. The 85 nematodes collected from Group II, presented
mean intensity of 14.17?28.12, resulting in 4.72?9.39% of recovery from the total of
administered larvae. Non-statistical difference was noted regarding the total recovered
nematodes. It is possible to establish patent T. canis infection in adult dogs by administering
larvae recovered from tissues of experimentally infected mice. / O objetivo do presente estudo foi promover a infec??o experimental de c?es adultos atrav?s
da administra??o de larvas de T. canis obtidas de tecidos de camundongos experimentalmente
infectados. E, conseq?entemente, avaliar a infec??o patente, determinando o per?odo pr?patente
(PPP) e o n?mero de nemat?ides recuperados em rela??o as diferentes quantidades de
larvas infectantes administradas. F?meas de T. canis foram coletadas de animais naturalmente
infectados, estas foram dissecadas para obten??o dos ovos e posterior cultura em solu??o de
formalina a 2%, em estufa climatizada do tipo B.O.D. ? temperatura de 27?1? C, com
umidade relativa de 75 ?10%. Ap?s 60 dias, camundongos foram infectados com os ovos
embrionados oriundos da cultura. Os roedores foram eutanasiados 26 dias p?s-infec??o (dpi),
submetidos ? necropsia. Seus ?rg?os e carca?as submetidos ? T?cnica de Isolamento ?cido
para recupera??o das larvas, as quais foram subdivididas em al?quotas para infec??o dos c?es.
Foram utilizados 12 c?es machos adultos da ra?a Beagle, pertencentes ao Canil do
Laborat?rio de Quimioterapia Experimental em Parasitologia Veterin?ria do Departamento de
Parasitologia Animal, Instituto de Veterin?ria da Universidade Federal Rural do Rio de
Janeiro. Os animais eram comprovadamente livres de infec??o pelo nemat?ide, por?m com
pr?vio hist?rico de parasitismo pelo nemat?ide em quest?o. Os animais foram divididos em
dois grupos de seis animais cada. Sendo os animais do Grupo I infectados com 60 larvas e, os
pertencentes ao Grupo II com 300 larvas, sendo estas recuperadas apenas dos c?rebros e
carca?a dos hospedeiros parat?nicos. Para avalia??o do per?odo pr?-patente foram empregadas
em associa??o, as t?cnicas coproparasitol?gicas de Centr?fugo-flutua??o Simples,
Sedimenta??o Simples e Sedimento-centr?fugo-flutua??o nos dias +8, + 12 e,
consecutivamente entre os dias +16 e + 56. Para recupera??o dos nemat?ides os animais
foram vermifugados nos dias +57, +58 e +59 com um produto a base de piperazina. Os
animais do Grupo I atingiram o PPP, em m?dia, aos 48 ?5,37dpi, j? os do Grupo II aos
41,5?5,09 dpi, havendo diferen?a estat?stica entre os per?odos (p<0,05). Foram recuperados
ao todo dez nemat?ides dos animais do Grupo I, com uma intensidade m?dia de 1,66?1,63,
resultando num total de recupera??o de 2,78?2,73%. Enquanto, no Grupo II, foi recuperado
um total de 85 helmintos, com uma intensidade m?dia de 14,17?28,12 parasitos por animal,
perfazendo 4,72?9,39% do total de larvas administradas. N?o havendo diferen?a significativa
entre os grupos em rela??o ao total de recupera??o de nemat?ides. ? poss?vel estabelecer infec??o patente em c?es adultos a partir da administra??o das diferentes cargas de larvas
recuperadas de tecidos de camundongos experimentalmente infectados.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/757 |
| Date | 25 February 2008 |
| Creators | Verocai, Guilherme Gomes |
| Contributors | Scott, Fabio Barbour, Grisi, Laerte |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Curso de P?s-Gradua??o em Ci?ncias Veterin?rias, UFRRJ, Brasil, Parasitologia Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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