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Previous issue date: 2009-02-27 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Experiments were carried out to adapt the technical photodynamic therapy (PDT) to the
subclinical mastitis treatment in bovine. Two LEDs equipments were tested with
manufactured acrylic fibers to penetration in the teats. Different energy densities were tested
to development the methodology. The photodynamic therapy using low energy density was
efficient to treat subclinical mastitis, it can be seen the reduction of mammary infection. The
constructions the acrylic fibers to LEDs equipments were efficient, where it was possible to
reach all interior of the mammary treated. The toluidine blue at 2,5% was efficient to light
dispersal and in microbial activity. The energy densities (ED) used was efficient to the
principal microbial agents of subclinical mastitis found in the present work. Staphylococcus
coagulase positive was removed with ED 25J/cm2, Staphylococus coagulase negative were
removed with ED 75J/cm2, Bacillus sp was removed with ED 100J/cm2, Streptococcus
dysgalactiae bacterial total count was reduced when ED 200J/cm2 was used. / Foram realizados v?rios experimentos para adequar ? t?cnica terapia fotodin?mica (PDT) para
o tratamento de mastite subcl?nica em bovinos. Foram avaliados dois aparelhos de LEDs
adaptados com fibra de acr?lico para a penetra??o nos tetos. Varias densidades de energia
foram testadas para o efeito desejado com o desenvolvimento de uma metodologia pr?pria. A
terapia fotodin?mica utilizando baixa densidade de energia para tratar mastite subcl?nica foi
eficiente posto que, houve redu??o na infec??o nos quartos mam?rios tratados. As adapta??es
para adequa??o dos aparelhos LEDs para tratamento dos tetos afetados com mastite subcl?nica
em bovinos foi eficiente permitindo que a irradia??o atingisse todo interior do quarto mam?rio
tratado. O agente fotossensibilizante azul de toluidina a 2,5% foi capaz de desencadear o
efeito desejado permitindo a dispers?o da luz e estimulando resposta antimicrobiana. As
densidades de energia (DE) testadas reduziram os principais agentes microbianos isolados.
Staphylococcus coagulase positivos foi eliminado com DE 25J/cm2, Estafilococos coagulase
negativos com DE 75J/cm2, Bacillus sp. com DE 100J/cm2 e Streptococcus dysgalactiae
diminuiu na contagem bacteriana total quando se utilizou DE 200J/cm2.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/839 |
| Date | 27 February 2009 |
| Creators | Silva, L?via Helena Moreira da |
| Contributors | Souza, Jos? Carlos Pereira de |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Curso de P?s-Gradua??o em Ci?ncias Veterin?rias, UFRRJ, Brasil, Parasitologia Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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