Made available in DSpace on 2016-04-28T20:16:29Z (GMT). No. of bitstreams: 1 2009 - Livia Helema Moreira da Silva.pdf: 9133264 bytes, checksum: 7ce4e3195e33e4e4dd04e40229f544bb (MD5) Previous issue date: 2009-02-27 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Experiments were carried out to adapt the technical photodynamic therapy (PDT) to the subclinical mastitis treatment in bovine. Two LEDs equipments were tested with manufactured acrylic fibers to penetration in the teats. Different energy densities were tested to development the methodology. The photodynamic therapy using low energy density was efficient to treat subclinical mastitis, it can be seen the reduction of mammary infection. The constructions the acrylic fibers to LEDs equipments were efficient, where it was possible to reach all interior of the mammary treated. The toluidine blue at 2,5% was efficient to light dispersal and in microbial activity. The energy densities (ED) used was efficient to the principal microbial agents of subclinical mastitis found in the present work. Staphylococcus coagulase positive was removed with ED 25J/cm2, Staphylococus coagulase negative were removed with ED 75J/cm2, Bacillus sp was removed with ED 100J/cm2, Streptococcus dysgalactiae bacterial total count was reduced when ED 200J/cm2 was used. / Foram realizados v?rios experimentos para adequar ? t?cnica terapia fotodin?mica (PDT) para o tratamento de mastite subcl?nica em bovinos. Foram avaliados dois aparelhos de LEDs adaptados com fibra de acr?lico para a penetra??o nos tetos. Varias densidades de energia foram testadas para o efeito desejado com o desenvolvimento de uma metodologia pr?pria. A terapia fotodin?mica utilizando baixa densidade de energia para tratar mastite subcl?nica foi eficiente posto que, houve redu??o na infec??o nos quartos mam?rios tratados. As adapta??es para adequa??o dos aparelhos LEDs para tratamento dos tetos afetados com mastite subcl?nica em bovinos foi eficiente permitindo que a irradia??o atingisse todo interior do quarto mam?rio tratado. O agente fotossensibilizante azul de toluidina a 2,5% foi capaz de desencadear o efeito desejado permitindo a dispers?o da luz e estimulando resposta antimicrobiana. As densidades de energia (DE) testadas reduziram os principais agentes microbianos isolados. Staphylococcus coagulase positivos foi eliminado com DE 25J/cm2, Estafilococos coagulase negativos com DE 75J/cm2, Bacillus sp. com DE 100J/cm2 e Streptococcus dysgalactiae diminuiu na contagem bacteriana total quando se utilizou DE 200J/cm2.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-08-15T18:23:07Z No. of bitstreams: 1 2009 - L?via Helena Moreira da Silva.pdf: 9761965 bytes, checksum: 0fa6815cc4bc10c43d8eea0b989dc84d (MD5) / Made available in DSpace on 2017-08-15T18:23:07Z (GMT). No. of bitstreams: 1 2009 - L?via Helena Moreira da Silva.pdf: 9761965 bytes, checksum: 0fa6815cc4bc10c43d8eea0b989dc84d (MD5) Previous issue date: 2009-02-27 / CNPq / Experiments were carried out to adapt the technical photodynamic therapy (PDT) to the subclinical mastitis treatment in bovine. Two LEDs equipments were tested with manufactured acrylic fibers to penetration in the teats. Different energy densities were tested to development the methodology. The photodynamic therapy using low energy density was efficient to treat subclinical mastitis, it can be seen the reduction of mammary infection. The constructions the acrylic fibers to LEDs equipments were efficient, where it was possible to reach all interior of the mammary treated. The toluidine blue at 2,5% was efficient to light dispersal and in microbial activity. The energy densities (ED) used was efficient to the principal microbial agents of subclinical mastitis found in the present work. Staphylococcus coagulase positive was removed with ED 25J/cm2 removed with ED 75J/cm2, Staphylococus coagulase negative were , Bacillus sp was removed with ED 100J/cm2 , Streptococcus dysgalactiae bacterial total count was reduced when ED 200J/cm2 was used. / Foram realizados v?rios experimentos para adequar ? t?cnica terapia fotodin?mica (PDT) para o tratamento de mastite subcl?nica em bovinos. Foram avaliados dois aparelhos de LEDs adaptados com fibra de acr?lico para a penetra??o nos tetos. Varias densidades de energia foram testadas para o efeito desejado com o desenvolvimento de uma metodologia pr?pria. A terapia fotodin?mica utilizando baixa densidade de energia para tratar mastite subcl?nica foi eficiente posto que, houve redu??o na infec??o nos quartos mam?rios tratados. As adapta??es para adequa??o dos aparelhos LEDs para tratamento dos tetos afetados com mastite subcl?nica em bovinos foi eficiente permitindo que a irradia??o atingisse todo interior do quarto mam?rio tratado. O agente fotossensibilizante azul de toluidina a 2,5% foi capaz de desencadear o efeito desejado permitindo a dispers?o da luz e estimulando resposta antimicrobiana. As densidades de energia (DE) testadas reduziram os principais agentes microbianos isolados. Staphylococcus coagulase positivos foi eliminado com DE 25J/cm2, Estafilococos coagulase negativos com DE 75J/cm2, Bacillus sp. com DE 100J/cm2 e Streptococcus dysgalactiae diminuiu na contagem bacteriana total quando se utilizou DE 200J/cm