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Previous issue date: 2006-03-31 / The present work was carried out in three different goat raisings and 27 females and 2 males
were selected for identifying the present microbiota in the preputial and vaginal cavity of
goats under three estrous cycle phases: In Februrary, 2005 in the pro-estrum, in May, 2005 in
the post-copula and in October, 2005 in the post-partum. The samples were collected by
swabs as well as vaginal and preputial washings for studying microbiota and by gynecological
brushes for the females esfoliative cytological study. The two greatest bacteria genera
prevalence were: Micrococcus spp (89,65 %) following by Bacillus spp. (29,90 %).
Filamentous fungi as Cladosporium spp. (26,39 %) and Aspergillus spp. (11,46 %) were the
most isolated. On the bacteria cytological test about 76 % of the investigations have been in
accordance with the microscopic finding and bacteria isolation. In 89% appraised animal
some yeasts presence on sheets was observed in spite of there was isolation just in 7,4 % of
the animals. There was similarity between hifas observation and filamentous fungi isolation,
in regarding to 53 % of hifas presence investigations. The bacterial and fungical microbiota
found on this survey showed the goats vaginal and preputial microbiota diversity and pointed
out to the fact that it might be carried during copulation. The number of isolated bacteria
species in the post-copulation phase was upper, as well as for yeasts. The vaginal cytology
performed significative association in relation to the bacterial isolation, observed by cocos
and bacilli presence on the sheets with positive growth. Bacteria potentially pathogenic as
Pseudomonas alcalinigenes, P. aeruginosa, and Corynebacterium spp. as well as opportunist
fungi as Aspergillus flavus in healthy goats vaginal cavity were found. / O presente trabalho foi desenvolvido em tr?s criat?rios caprinos distintos, de onde tomamos
27 f?meas e 2 machos a fim de identificar a microbiota presente no prep?cio de bodes e na
cavidade vaginal de cabras em tr?s fases do ciclo estral: fevereiro de 2005 no proestro, maio
de 2005 ap?s a c?pula e outubro de 2005 ap?s o parto. As amostras foram coletadas atrav?s
de swab, lavado vaginal e prepucial para o estudo da microbiota e com escovas ginecol?gicas
para o estudo colpocitol?gico das f?meas. Os dois g?neros de bact?rias de maior preval?ncia
foram os seguintes: Micrococcus spp. (89,65 %) e Bacillus spp. (29,90 %). Os dois g?neros de
fungos de maior preval?ncia foram Cladosporium spp. (26,39 %) e Aspergillus spp. (11,46
%). No estudo colpocitol?gico para bact?rias, em 76 % das investiga??es houve concord?ncia
entre os achados microsc?picos e isolamento bacteriano. Em 89 % dos animais avaliados foi
observada a presen?a de leveduras nas l?minas, mas houve isolamento em apenas 7,4 % dos
animais. Em 53 % das investiga??es da presen?a de hifas houve concord?ncia entre a
observa??o de hifas e o isolamento de fungos filamentosos. A microbiota f?ngica e bacteriana
encontrada no presente trabalho evidenciou a diversidade da microbiota vaginal e prepucial de
caprinos e aponta para o fato de que podem ser carreados durante a c?pula. Observou-se que o
n?mero de esp?cies isoladas de bact?rias ? superior na fase de p?s-c?pula enquanto que em
fungos observamos este aumento no p?s-parto; O resultado da citologia vaginal teve
correla??o com o isolamento bacteriano, observada pela presen?a de cocos e bacilos nas
l?minas com crescimento bacteriano positivo; Bact?rias potencialmente patog?nicas como
Pseudomonas alcaligenes, P. aeruginosa e Corynebacterium spp. foram encontrados na
cavidade vaginal de cabras h?gidas, assim como fungos oportunistas como Aspergillus flavus.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/856 |
| Date | 31 March 2006 |
| Creators | Gomes, Marcelo Carvalho |
| Contributors | Direito, Gl?ria Maria, Fraga, Marcelo Elias |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Microbiologia Veterin?ria, UFRRJ, Brasil, Microbiologia Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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