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Previous issue date: 2017-07-04 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / A sepse e uma disfuncao organica causada por uma resposta imune desregulada a
uma infeccao. A mesma tem em sua abordagem terapeutica inicial a utilizacao de
antimicrobianos de amplo espectro, os quais nao sao suficientes para o controle da
infeccao, sendo necessario a busca por novas terapias focadas na interrupcao da
resposta inflamatoria em cascata produzida pela invasao e disseminacao de agentes
patogenicos. Dessa forma, a saliva do Aedes aegypti apresenta caracteristicas
imunomoduladoras, com potencial de inibicao de citocinas pro-inflamatorias, bem
como a presenca de peptideos ativadores de oxido nitrico. Com isso, seria de grande
interesse investigar o efeito imunomodulador da saliva em modelo animal de sepse.
Neste sentido, camundongos foram pre-tratados com a saliva do Aedes aegypti sendo
a sepse induzida pelo modelo de transfixacao do coto-cecal. Apos 12 e 24 horas as
amostras foram coletadas, e avaliado a sobrevida, carga bacteriana, migracao
leucocitaria e parametros oxidativo (NO, EROs, MDA, TEAC, DPPH e GSH). A saliva
melhorou o prognostico dos animais, aumentando a sobrevida e o peso. Alem disso,
reduziu a carga bacteriana e aumentou o influxo de monocitos no sangue. A saliva,
tambem, mostrou efeito antioxidante por reduzir a producao de especies reativas e
aumentar a capacidade antioxidante, alem de diminuir a peroxidacao lipidica. Assim,
a saliva foi capaz de inibir os danos causados pela sepse em animais in vivo
melhorando seu prognostico. / Sepsis is an organ dysfunction caused by a dysregulated immune response to an
infection, the initial therapeutic approach to sepsis are broad spectrum antimicrobials,
which is not sufficient for control of infection, requiring association with other therapies
focused in the interruption of the inflammatory response chain, provided by the
pathogens. In this way, the Aedes Aegypti’s saliva presents immunomodulary features,
with a potential pro inflammatory cytokine inhibition, as well as the presence of nitric
oxide peptides activators. Therefore, it would be a great interest to search the saliva’s
immunomodulator effect in animal model sepses. In this regards, mices were pre
treated with Aedes aegypti saliva, and sepsis was induced by the cecal ligation and
puncture. After 12 and 24 hours, the samples were collected, and evaluated the
survival rate, bacteria level, leukocyte migration, and oxidative parameters (NO, EROs,
MDA e TEAC). The saliva improved the animal prognostic, increasing the survival rate
and weight. Furthermore, decreased the bacterial levels and increased the influx of
monocytes. The saliva, in addition, presented antioxidant effects by reducing
production of the reatives species, and increasing the antioxidant capacity, other than
decrease the lipid peroxidation. Thus, the saliva was capable to inhibit damages
caused by sepsis in animals in vivo, improving its prognostic.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufpa.br:2011/10552 |
| Date | 04 July 2017 |
| Creators | GOMES, Rafaelli de Souza |
| Contributors | MONTEIRO, Marta Chagas |
| Publisher | Universidade Federal do Pará, Programa de Pós-Graduação em Ciências Farmacêuticas, UFPA, Brasil, Instituto de Ciências da Saúde |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | 1 CD-ROM, reponame:Repositório Institucional da UFPA, instname:Universidade Federal do Pará, instacron:UFPA |
| Rights | info:eu-repo/semantics/openAccess |
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