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Previous issue date: 2012-12-10 / Obesity is increasing, reaching epidemic levels in many regions of the world. Studies
have shown that consumption of peanuts influences on weight control and this
influence may be due to the action of trypsin inhibitors sacietog?nica that condition
increased plasma colescistocinina (CCK). Moreover, the peanut has other health
benefits, and these assignments are guaranteed to increase their production and
consumption of several of its products, including the pa?oca peanut. The aim of this
study was to identify the presence of a trypsin inhibitor in pa?oca peanut and
evaluate its effect on food intake, weight gain and histomorphological changes in
swiss mice (n = 8) and Wistar rats (n = 6). Experimental diets were prepared based
on the AIN-93G and supplemented with tack or peanut trypsin inhibitor partially
purified pa?oca peanut (AHTI). After each treatment, the animals were anesthetized
and euthanized, their bloods were collected by cardiac puncture for the determination
of CCK and other biochemical parameters (glucose, triglycerides, total cholesterol,
high density lipoprotein, low density lipoprotein, glutamic-pyruvic transaminase,
glutamic oxaloacetic transaminase and albumin) and their pancreas removed for
histologic and morphometric analysis. The supplementation with pa?oca peanut and
the AHTI showed a decrease of body weight gain and food intake in both mice and
rats, due to the satiety, since the animals showed no evidence of impairment of
nutritional status conditioned by consumption the AHTI. There were also observed
biochemical or morphological important when compared with controls. However,
AHTI led to increased secretion of CCK, a peptide sacietog?nico. Thus, these results
indicate that AHTI present in pa?oca peanut, is able to enhance the secretion of
plasma CCK and thereby reduce the weight gain associated with lower food intake of
experimenta animals / A obesidade ? crescente, atingindo n?veis epid?micos, em muitas regi?es do
mundo. Estudos t?m mostrado que o consumo de amendoins influencia no controle
de peso e essa influ?ncia pode ser devido ? a??o sacietog?nica de inibidores de
tripsina que condicionam o aumento plasm?tico de colecistocinina (CCK). Al?m
disso, o amendoim apresenta outros benef?cios ? sa?de e essas atribui??es t?m
garantido o aumento da sua produ??o e o consumo de v?rios de seus produtos,
entre eles, a pa?oca de amendoim. O objetivo deste estudo foi identificar a presen?a
de um inibidor de tripsina na pa?oca de amendoim e avaliar o seu efeito sobre o
consumo alimentar, o ganho de peso e altera??es histomorfol?gicas em
camundongos swiss (n=8) e ratos wistar (n=6). Dietas experimentais foram
preparadas com base na AIN-93G e suplementadas com a pa?oca de amendoim ou
com o inibidor de tripsina parcialmente purificado da pa?oca de amendoim (AHTI).
Ao final de cada tratamento, os animais foram anestesiados, eutanasiados e tiveram
seus sangues colhidos, por pun??o card?aca, para a dosagem de CCK e de outros
par?metros bioqu?micos (glicose, triglicer?deos, colesterol total, lipoprote?nas de altadensidade,
lipoprote?na de baixa-densidade, transaminase glut?mica pir?vica,
transaminase glut?mica oxalac?tica e albumina) e seus p?ncreas extra?dos para
an?lise histol?gica e morfom?trica. As suplementa??es com a pa?oca de amendoim
e com o AHTI mostraram uma diminui??o do ganho de peso corporal e do consumo
alimentar tanto em camundongos quanto em ratos, devido ? saciedade, uma vez
que os animais n?o apresentaram ind?cios de comprometimento do estado
nutricional condicionada pelo consumo do AHTI. N?o foram, tamb?m, observadas
altera??es bioqu?micas, nem histomorfol?gicas importantes quando comparadas
com os controles. No entanto, o AHTI levou ao aumento da secre??o de CCK, um
pept?dio sacietog?nico. Assim, estes resultados sugerem que o AHTI, presente na
pa?oca de amendoim, seria capaz de aumentar a secre??o plasm?tica de CCK e
reduzir o ganho de peso associado com menor consumo alimentar de animais
experimentais
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/12601 |
| Date | 10 December 2012 |
| Creators | Serquiz, Alexandre Coelho |
| Contributors | CPF:41305655400, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782221T9&dataRevisao=null, Rezende, Adriana Augusto de, CPF:05410395808, http://lattes.cnpq.br/4245215108740331, Lima, Liziane Maria de, CPF:73747211453, http://lattes.cnpq.br/7350753350522784, Santos, Elizeu Antunes dos |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Bioqu?mica, UFRN, BR, Bioqu?mica; Biologia Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/embargoedAccess |
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