Made available in DSpace on 2014-12-17T14:10:22Z (GMT). No. of bitstreams: 1
SimoneCL_DISSERT.pdf: 2150980 bytes, checksum: 9a39148c92d0ec9032d34b53daffd92a (MD5)
Previous issue date: 2009-09-28 / The brazilian-plum (Spondias tuberosa, His) is a tropical fruit tree that has been consolidated
in the market for agribusiness processing, due to its characteristic flavor of fruit. Accordingly,
studies to optimize the propagation of plants are necessary for production of seedlings with
agronomic and quality assurance measures. This study aimed at determining the efficient
techniques for uniform seed germination, as brazilian-plum seed present mechanical
dormancy, and establish optimal culture media for multiplication of shoots from the in vitro
micropropagation. Firstly, in a greenhouse at the Universidade Federal do Rio Grande do
Norte, was evaluated the influence of different methods of breaking dormancy in the
emergence of seedlings of brazilian-plum and speed of germination (IVG) of seeds. After 60
days of cultivation, it was found that splay in the distal portion of the seed was the best
treatment, with rates of 85.33% in germinability and 3.415 of IVG, compared with the
treatment of seed-soaking in water for 12h + humus and the control group. Subsequently, new
sources of seedling explants were obtained in studies of tissue culture. Laboratory of Plant
Biotechnology that the university, was used stem apex, nodal segments and internodes in
search of decontamination with various concentrations of calcium hypochlorite [Ca(OCl)2]
and micropropagation, inoculating them in half WPM (1980) with various concentrations of
6-benzylaminopurine (BAP). We used 10 sample units with three replications for different
concentrations of [Ca(OCl)2], BAP and explants type. After thirty days, which was observed
for the control of contamination, during the establishment in vitro, concentrations of
[Ca(OCl)2] between 0.5% and 2.0% were effective in combating exogenous contamination of
the apex. In nodal segments and internodes, concentrations of [Ca(OCl)2] between 1.0% and
2.0% and 1.5% and 2.0% were respectively, sufficient to reduce the percentage of losses in
these infestations explants. For micropropagation, the culture medium supplemented with 0.1
mg.L-1 BAP promotes better development of multiple shoots per explants from nodal
segment. However, success does not get to shoot training in internodal segment / O umbuzeiro (Spondias tuberosa, Arr.) ? uma ?rvore frut?fera tropical que vem se
consolidando no mercado das agroind?strias de processamento, devido ao seu fruto de sabor
caracter?stico. Nesse sentido, estudos para otimizar a propaga??o de plantas s?o necess?rios
para garantir a produ??o de mudas com qualidade agron?mica e garantias fitossanit?rias.
Assim, este trabalho teve como finalidade determinar as t?cnicas eficientes para uniformizar a
germina??o, j? que as sementes de umbuzeiro apresentam dorm?ncia mec?nica, e estabelecer
meios de cultura ideais para a multiplica??o de brotos, bem como reduzir as contamina??es e
oxida??es in vitro. Primeiramente, em casa de vegeta??o da Universidade Federal do Rio
Grande do Norte, foi avaliado a influ?ncia de diferentes m?todos de quebra de dorm?ncia na
emerg?ncia de pl?ntulas de umbuzeiro e na velocidade de germina??o (IVG) de sementes.
Ap?s 60 dias de cultivo, verificou-se que o corte em bisel na por??o distal da semente foi o
melhor tratamento, com ?ndices de 85,33% de germinabilidade e 3,415 de IVG, quando
comparado com o tratamento de embebi??o em ?gua + h?mus por 12h e o grupo controle.
Posteriormente, as novas pl?ntulas obtidas foram fontes de explantes em trabalhos de cultura
de tecidos. No Laborat?rio de Biotecnologia Vegetal dessa mesma institui??o de ensino, se
utilizou ?pices caulinares, segmentos nodais e internodais em pesquisas de descontamina??o,
com variadas concentra??es de hipoclorito de c?lcio [Ca(OCl)2] e de micropropaga??o,
inoculando-os em meio WPM (1980) com variadas concentra??es de 6-benzilaminopurina
(BAP). Utilizou-se 10 unidades amostrais com tr?s repeti??es para as diferente concentra??es
de [Ca(OCl)2], de BAP e tipo de explante. Ao fim de trinta dias, se observou que para o
controle da contamina??o, durante o estabelecimento in vitro, as concentra??es de [Ca(OCl)2]
entre 0,5% e 2,0% foram eficientes no combate das contamina??es ex?genas do ?pice
caulinar. Nos segmentos nodais e internodais, as concentra??es de [Ca(OCl)2] entre 1,0% e
2,0% e 1,5% e 2,0% foram respectivamente, suficientes na diminui??o do percentual de
perdas por infesta??es nestes explantes. Para a micropropaga??o, o meio de cultura
suplementado com 0,1 mg.L-1 BAP favorece o melhor desenvolvimento de brotos m?ltiplos
por explante a partir de segmento nodal. Entretanto, n?o se obt?m sucesso de forma??o de
brotos em segmento internodal
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13061 |
| Date | 28 September 2009 |
| Creators | Lima, Simone Cassiano de |
| Contributors | CPF:46609954453, http://lattes.cnpq.br/4153117506852558, Lameira, Osmar Alves, CPF:03337189253, http://lattes.cnpq.br/2428151792713626, Chellappa, Sathyabama, CPF:15644812472, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787789Y9, Aloufa, Magdi Ahmed Ibrahim |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias Biol?gicas, UFRN, BR, Biodiversidade; Biologia Estrutural e Funcional. |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
Page generated in 0.0062 seconds