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Previous issue date: 2007-02-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Some microorganisms from virgin ecosystems are able to use petroleum it as source of carbon and energy. The knowledge of microbial biodiversity can help to reveal new metabolic systems for utilization alkanes with biotechnological
importance. The aim of this study is: i) Accomplish an in silico study of the AlkB protein aimed to understand the probable mechanism involved on selectivity of alkanes in Gram positive and Gram negative bact?ria. ii) prospect and analyze
the response of the microbial alkanotrophics communities in soil and mangrove sediments of BPP RN and soil of Atlantic forest in the Horto Dois Irm?os Reserve area/PE using the molecular biomarker, gene alkB; with the PCR and
PCR-DGGE approach / Alguns microrganismos de ecossistemas virgens s?o capazes de utilizar petr?leo como fonte de carbono e energia. O conhecimento dessa biodiversidade microbiana pode revelar vias metab?licas microbianas para utilizar alcanos que venham a ter import?ncia biotecnol?gica. Os objetivos do presente trabalho s?o: i) realizar estudos in silico da prote?na AlkB visando obter subs?dios para a compreens?o do mecanismo de seletividade na utiliza??o de alcanos em bact?rias Gram positivas e Gram negativas; ii) prospectar e avaliar a resposta de comunidades microbianas alcanotr?ficas de solos e mangue da BPP RN e solo da Mata Atl?ntica na Reserva Horto Dois
Irm?os-PE utilizando o gene alkB como biomarcador molecular, e as t?cnicas de PCR e PCR-DGGE
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/16777 |
| Date | 28 February 2007 |
| Creators | Pereira, Matheus Silva |
| Contributors | CPF:80573380082, http://lattes.cnpq.br/2307806644081146, Ara?jo, Magn?lia Fernandes Flor?ncio de, CPF:51274957400, http://lattes.cnpq.br/4511989641459455, Oliveira, Val?ria Maia de, CPF:11943438803, http://lattes.cnpq.br/3886687872358496, Blaha, Carlos Alfredo Galindo |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Gen?tica e Biologia Molecular, UFRN, BR, Gen?tica e Biologia Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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