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Previous issue date: 2015-02-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / A esp?cie Rachycentron canadum, conhecida popularmente como beijupir? ou cobia, ? o
?nico representante da fam?lia Rachycentridae o qual vem sendo crescentemente utilizado
na piscicultura marinha, em cultivos intensivos. Como caracter?sticas vantajosas, ? de f?cil
adapta??o, prol?fica, possui crescimento precoce em cativeiro e elevado valor comercial. J?
as esp?cies da fam?lia Lutjanidae (Lutjanus synagris, Lutjanus jocu, Lutjanus analis, Lutjanus
alexandrei e Ocyurus chrysurus) representam um importante recurso pesqueiro em todas as
?reas de sua ocorr?ncia. No Brasil a explora??o comercial dos Lutjanidae que se iniciou na
d?cada de 60 e nos anos 80 j? demonstrava um decl?nio nos volumes de captura. Este fato
aponta que os lutjan?deos devem possuir um manejo conservativo. Apesar dos potencias
econ?micos, pouco se conhece sobre as caracter?sticas gen?ticas e citogen?ticas destas
esp?cies, principalmente no que diz respeito a an?lise de DNAs repetitivos, os quais que
representam a maior parte do genoma dos eucariotos e sendo responsaveis por importantes
papeis evolutivos no genoma dos peixes. Dados citogen?ticos vem crescentemente sendo
empregados em estudos populacionais e com fins biotecnol?gicos em peixes. As analises
citogen?ticas foram realizadas utilizando m?todos cl?ssicos como colora??o com Giemsa,
bandamento C e Ag-RONs, colora??o com os fluorocromos base-espec?ficos (DAPI e MM) e
mapeamento cromoss?micos de sequ?ncias repetitivas dentre as quais, sequ?ncias
telom?ricas, transposons (Tol2), retrotransposons (Rex1 e Rex3), DNA repetitivos (Cot-1 e
microssat?lites) e das regi?es transcricionalmente ativas dos genes ribossomais 18S e 5S e
histonas (H2BA e H3), atrav?s da hibrida??o in situ com sondas fluorescentes (FISH). Os
padr?es cromoss?micos obtidos contribu?ram para o conhecimento da organiza??o das
sequ?ncias repetitivas no genoma das esp?cies, bem como a diferencia??o cariot?pica.
Padr?es incomuns de expans?o de sequ?ncias hist?nicas retratam a primeira ocorr?ncia em
peixes marinhos. Os dados obtidos fornecem subs?dios para o conhecimento gen?tico dos
importantes recurso pesqueiros representados pelas esp?cies aqui analisadas, com vistas a
auxiliar o desenvolvimento da piscicultura marinha. / The Rachycentron canadum species, commonly known as beijupir? or cobia is the only
representative of Rachycentridae family which has been increasingly used in marine fish
farming, in intensive cultivation. As advantageous features it has easy adaptation, prolific
behavior, early growth in captivity and high commercial value. Additionally, specie of
Lutjanidae family (Lutjanus synagris, Lutjanus jocu, Lutjanus analis, Lutjanus alexandrei and
Ocyurus chrysurus) represents an important fisheries resource in all areas of its occurrence.
In Brazil, the commercial exploitation of Lutjanidae which begun in the 60's and 80's, already
has showed a decline in catch volumes. This fact suggests that the snappers must have a
conservative management. Despite the economic potential, little is known about the genetic
and cytogenetic characteristics of these species, especially with respect to repetitive DNA
analysis, which represents the major part of the eukaryotes genome, playing important
evolutionary roles in the fish genome. Cytogenetic data is increasingly being used in
population studies and biotechnological purposes in fishes. The cytogenetical analyzes were
performed using classical methods such as Giemsa staining, C-banding and Ag-NORs,
fluorochromes base-specific staining (DAPI and MM) and physical mapping of repetitive
sequences among which, telomeric sequences, transposons (Tol2), retrotransposons (Rex1
and Rex3), repetitive DNA (microsatellites and Cot-1) and transcriptionally active regions of
the 18S and 5S ribosomal genes and histone (H3 and H2BA) by in situ hybridization with
fluorescent probes (FISH). The chromosomal patterns obtained contributed to the
organization of repetitive sequences in the genome of the species, as well as karyotypical
differentiation. Unusual patterns of histone sequences expansion depict the first occurrence
in marine fishes. The obtained data provided subsides to the genetic knowledge of the
important fisheries resource represented by the species here analyzed, seeking the marine
pisciculture improvement.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20109 |
| Date | 27 February 2015 |
| Creators | Costa, Gide?o Wagner Werneck F?lix da |
| Contributors | 56615043491, http://lattes.cnpq.br/8437464961129518, Scortecci, K?tia Castanho, 13451112825, http://lattes.cnpq.br/4808910380593455, Bertollo, Luiz Antonio Carlos, 03059677891, http://lattes.cnpq.br/7069073110550804, Cioffi, Marcelo de Bello, 07333907651, http://lattes.cnpq.br/0242034365085727, N?brega, Marcelo Francisco de, 14839619824, http://lattes.cnpq.br/6194914254799842, Molina, Wagner Franco |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM BIOTECNOLOGIA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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