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Previous issue date: 2016-02-26 / Les?es odontog?nicas epiteliais benignas s?o entidades de grande import?ncia cl?nica que se desenvolvem nos ossos maxilares a partir dos tecidos que formam os dentes. Tem sido demonstrado que em tumores benignos e malignos, est?o presentes um grande n?mero de c?lulas tronco tumorais, as quais tem grandes implica??es no desenvolvimento dos tumores. Oct-4 e CD44 t?m sido demostrados como importantes marcadores para c?lulas-tronco tumorais. O objetivo deste estudo foi identificar c?lulas epiteliais que expressam marcadores de c?lulas tronco atrav?s da express?o imuno-histoqu?mica de Oct-4 e CD44 em uma s?rie de casos de les?es odontog?nicas epiteliais ben?gnas. A amostra foi constitu?da por 20 casos de ceratocisto odontog?nico (CCO), 20 caos de Ameloblastoma s?lido/multic?stico e 20 casos de Tumor Odontog?nico Adenomatoide (TOA). A express?o de Oct-4 e CD44 foi avaliada no epit?lio das les?es atrav?s do percentual de c?lulas positivas(PP) e da intensidade da express?o ( IE ), sendo realizado o somat?rio destes escores, resultando na Pontua??o de Imunomarca??o Total (PIT) que variou de 0 a 7. Os resultados do presente estudo foram analisados pelo valor da pontua??o de PIT. Todos os casos apresentaram positividade para os dois marcadores e a maioria exibiu alta express?o para ambos os marcadores. A an?lise da express?o de Oct-4 n?o revelou diferen?as estatisticamente significativas (p = 0,406) entre as les?es estudadas. Com rela??o ? express?o do CD44, houve diferen?a estatisticamente significativa entre os casos de ameloblastoma e CCO, apresentando este ?ltimo maior n?mero de casos no score 7 (p = 0,034). Na analise da correla??o da imunoexpress?o de ambos os marcadores nas tr?s les?es estudadas, n?o houve correla??o estatisticamente significativa . Os resultados do presente estudo identificaram a presen?a de c?lulas com caracter?sticas troncais dispostas em locais variados do componente epitelial das les?es ora estudadas sugerindo a sua poss?vel participa??o na histog?nese e diferencia??o em les?es odontog?nicas epiteliais benignas contribuindo assim para o desenvolvimento destas les?es. / benign epithelial odontogenic lesions are great clinical importance entities that develop in the jaws from the tissues that form teeth. It has been shown that in benign and malignant tumors, are present in a large number of tumor stem cells, which has great implications in the development of these lesions. Oct-4 and CD44 have been demos as important markers for tumoral stem cells. The objective of this study was to identify epithelial cells expressing stem cell markers by immunohistochemical expression of Oct-4 and CD44 in a series of cases of benign epithelial odontogenic lesions. The sample was comprised of 20 cases of odontogenic keratocyst (OKC), 20 cases of solid/multicystic ameloblastoma and 20 cases of adenomatoid odontogenic tumor (AOT). The expression of Oct-4 and CD44 was evaluated in epithelial lesions using the percentage of positive cells (PP) and the intensity of expression (IE), being realized the sum of these scores, resulting in Total Immunostaining Score (TIS) ranging 0 to 7. The results were submitted to the appropriate statistical test (nonparametric Kruskal-Wallis and Spearman correlation coefficient). All cases were positive for both markers and most showed high expression of both markers. The analysis of Oct-4 expression revealed no statistically significant differences (p = 0.406) among the studied lesions. Regarding the CD44 expression, there was a statistically significant difference between the cases of ameloblastoma and TOA in relation to the CCO, with the latter show more cases in the score 7 (p = 0.034). In the correlation analysis of the immunoreactivity of both markers in the three lesions studied, there was no statistically significant correlation. The results of this study identified the presence of cells with stemness characteristics arranged at various sites in the epithelial component of the studied lesions suggesting their possible role in the histogenesis and differentiation in benign epithelial odontogenic lesions, thus contributing to the development of these lesions.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20948 |
| Date | 26 February 2016 |
| Creators | Monroy, Eduardo Alonso Cruz |
| Contributors | 08588724472, http://lattes.cnpq.br/6671914892609743, Nonaka, Cassiano Francisco Weege, 02781932426, http://lattes.cnpq.br/0224522010734716, Pinto, Le?o Pereira, 01608789420, http://lattes.cnpq.br/7040457616034306, Souza, Lelia Batista de |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM PATOLOGIA ORAL, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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