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Previous issue date: 2017-08-05 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / As infec??es oportunistas s?o reconhecidas como uma das complica??es mais comuns em pacientes imunocomprometidos, incluindo aqueles que s?o infectados pelo HIV, os quais est?o em maior risco de doen?a cr?nica e debilitante. Nesse contexto, dois cocc?deos intestinais, Cryptosporidium spp. e Cystoisospora spp., acometem pacientes HIV/AIDS e induzem quadros graves de diarreia e/ou um hist?rico de diarreia alternadamente com um intervalo assintom?tico. Portanto, o objetivo deste estudo foi avaliar a frequ?ncia de Cryptosporidium spp. e Cystoisospora spp. em pacientes internados com HIV/AIDS no Hospital Giselda Trigueiro, no per?odo de novembro de 2015 a mar?o de 2017. Neste estudo foi aplicado question?rio s?cio-econ?mico, feita consulta dos prontu?rios, e tamb?m foram colhidas 72 amostras fecais para investiga??o parasitol?gica pelos m?todos de concentra??o de Seather, Ritchie e Colora??o Ziehl-Neelsen modificado; bem como, detec??o de coproant?geno de Cryptosporidium spp. utilizando o ensaio imunoenzim?tico (ELISA). A amostra foi composta de 75% de homens e 25% de mulheres com idade variando entre 20 e 80 anos. A frequ?ncia de Cryptosporidium spp foi de 2,2%, atrav?s do ensaio imunoenzim?tico, e de Cystoisospora spp. foi de 6,9%, atrav?s dos m?todos de Ritchie e colora??o Zeehl-Neelsen modificado. Todos os pacientes faziam uso de Sulfametaxol e Trimetoprim e apresentavam no momento da coleta um quadro cl?nico de diarr?ia. Entre os pacientes positivos para estes cocc?deos, a contagem de linf?citos T CD4+ foi abaixo de 200 c?lulas/mm3 de sangue. / Opportunistic infections are recognized as one of the most common complications in immunocompromised patients, including those who are infected with HIV, who are at increased risk of chronic and illness.In this context, two intestinal coccidians, Cryptosporidium spp. and Cystoisospora spp., affect HIV / AIDS patients and induce severe diarrhea and / or a history of diarrhea alternately with an asymptomatic interval. Therefore, the objective of this study was to evaluate the frequency of Cryptosporidium spp. and Cystoisospora spp. in patients hospitalized with HIV/AIDS at the Hospital Giselda Trigueiro, between 2015 and 2017. In this study, a socioeconomic questionnaire was applied, and 72 fecal samples were collected for parasitological investigation using the methods of Seather, Ritchie and Modified Ziehl-Neelsen staining; as well as, detection of Cryptosporidium spp. coproantigen by enzyme-linked immunosorbent assay (ELISA). The sample consisted of 75% of men and 25% of women with ages ranging from 20 to 80 years. The frequency of Cryptosporidium spp. was 2.2%, through the immunoenzymatic assay, and Cystoisospora spp. was 6.9%, using Richie's methods and modified Ziehl-Neelsen staining. All patients used trimethoprim-sulfamethoxazole and presented diarrhea at the time of collection. Among the positive patients for these coccidians, the CD4 + T lymphocyte count was below 200 cells/mm3 of blood.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/24595 |
| Date | 05 August 2017 |
| Creators | Moura, Tenille Karinanna de Morais Paiva |
| Contributors | 59626305487, Oliveira, Carlos Capistrano Gon?alves de, 07496850420, Oliveira, Cl?udio Bruno Silva de, 06745139405, Souza, Maria de F?tima de, 32308698420, Andrade Neto, Valter Ferreira de |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS BIOL?GICAS, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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