Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Nucleotide- and nucleoside-degrading enzymes are present in the surface of platelets,
blood cells involved in clotting disturbances of Trypanosoma evansi-infected animals.
Thus, this study was aimed at evaluating the activity of the enzymes NTPDase, 5 -
nucleotidase and adenosine deaminase in platelets of rats experimentally infected by T.
evansi. Animals were divided into four groups, according to the degree of parasitemia.
Samples were collected at days 3 (group A: at the beginning of parasitemia), 5 (group B:
high parasitemia) and 15 (group C: chronic infection). Group D (control group) was
composed of non-infected animals. Blood samples with citrate as the anticoagulant were
collected and used for platelet separation and enzymatic assays. NTPDase, 5 -
nucleotidase and adenosine deaminase (ADA) activities were decreased (p<0.05) in
platelets from rats of groups A and B, when compared to the control group. In group C,
only NTPDase and 5 -nucleoside activities were decreased (p<0.001), observed by ADP
and AMP hydrolysis. The correlation between platelet count and nucleotide and
nucleoside hydrolysis was positive and statistically significant (p<0.05) in groups A and
B. Platelet aggregation of all infected groups was decreased in comparison to the
control group (p<0.05). Based upon the results, it is concluded that the alterations
observed in the activity of the enzymes NTPDase, 5 -nucleotidase and adenosine
deaminase in platelets of T. evansi-infected animals might be related to
thrombocytopenia. / Nucleotide- and nucleoside-degrading enzymes are present in the surface of platelets, blood cells involved in clotting disturbances of Trypanosoma evansi-infected animals.
Thus, this study was aimed at evaluating the activity of the enzymes NTPDase, 5 - nucleotidase and adenosine deaminase in platelets of rats experimentally infected by T. evansi. Animals were divided into four groups, according to the degree of parasitemia. Samples were collected at days 3 (group A: at the beginning of parasitemia), 5 (group B:
high parasitemia) and 15 (group C: chronic infection). Group D (control group) was composed of non-infected animals. Blood samples with citrate as the anticoagulant were collected and used for platelet separation and enzymatic assays. NTPDase, 5 - nucleotidase and adenosine deaminase (ADA) activities were decreased (p<0.05) in platelets from rats of groups A and B, when compared to the control group. In group C,
only NTPDase and 5 -nucleoside activities were decreased (p<0.001), observed by ADP and AMP hydrolysis. The correlation between platelet count and nucleotide and
nucleoside hydrolysis was positive and statistically significant (p<0.05) in groups A and B. Platelet aggregation of all infected groups was decreased in comparison to the
control group (p<0.05). Based upon the results, it is concluded that the alterations observed in the activity of the enzymes NTPDase, 5 -nucleotidase and adenosine
deaminase in platelets of T. evansi-infected animals might be related to thrombocytopenia.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufsm.br:1/10079 |
| Date | 12 August 2010 |
| Creators | Oliveira, Camila Belmonte |
| Contributors | Monteiro, Silvia Gonzalez, Teixeira, Marta Maria Geraldes, Mazzanti, Cinthia Melazzo Andrade |
| Publisher | Universidade Federal de Santa Maria, Programa de Pós-Graduação em Medicina Veterinária, UFSM, BR, Medicina Veterinária |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFSM, instname:Universidade Federal de Santa Maria, instacron:UFSM |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 500500000007, 400, 500, 500, 300, 500, f658c142-7522-4cd5-a133-f2479652bf31, 87419040-500d-4516-8802-ee0b3a74fcd0, 9599e866-721a-472e-b709-28b2298d7dcf, 06219c3e-9788-4345-8060-bf224f77f2e3 |
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