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gomes_ac_me_araca.pdf: 617778 bytes, checksum: dc3fa0c485a53f6482fbc321ee95103f (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O objetivo deste trabalho foi investigar os mecanismos envolvidos na migração de neutrófilos induzida pelo MTA para cavidade peritoneal de camundongos. Observouse que o MTA induziu migração de neutrófilos de maneira dose-dependente (0,5; 5; 50 e 100 mg/cavidade), alcançando o pico de migração 6 horas após a injeção do estímulo com a dose de 50 mg/cavidade. Esta migração foi parcialmente inibida pelo pré-tratamento dos animais com dexametasona (1 mg/Kg), BWA4C (50 mg/Kg) e U75302 (0,5 mg/Kg). Diferentemente, a Indometacina (5 mg/Kg) foi inefetiva neste processo. Verificou-se também que os animais estimulados com MTA (50 mg/cavidade) apresentaram uma liberação significativa de IL-1ß e MIP-2 no exsudato peritoneal. O pré-tratamento com Tioglicolato aumentou em cerca de 380% a população de macrófagos na cavidade peritoneal, potencializando a migração de neutrófilos induzida pelo MTA (p<0,05). O pré-tratamento com composto 48/80 depletou cerca de 75% a população de mastócitos, diminuindo a migração de neutrófilos (p<0,05). A injeção de MTA na bolha de ar subcutânea induziu uma migração de neutrófilos menor comparada à cavidade peritoneal. Estes resultados confirmam a participação de mastócitos e macrófagos na migração de neutrófilos induzida pelo MTA. A injeção de sobrenadante de macrófagos e mastócitos estimulados com MTA na cavidade peritoneal de camundongos causou significante migração de neutrófilos (p<0,05), que foi parcialmente inibida pelo pré-tratamento das células por dexametasona (10 æMolar), BWA4C (100 æMolar) e U75302 (10 æMolar) sugerindo a liberação por essas células de LTB4 e citocinas e/ou quimiocinas. Confirmando esses dados,... / The aim of this study was to investigate the mechanism involved in the neutrophil migration induced by MTA into peritoneal cavity in mice. It was observed that MTA induced a dose dependent neutrophil migration (0.5, 5, 50 and 100 mg/cavity), achieving the peak 6 hours after the stimulation with 50 mg/cavity. Neutrophil migration was inhibited by the pre-treatment with dexamethasone (1 mg/Kg), BWA4C (50 mg/Kg) and U75302 (0,5 mg/Kg). Differently indometacin (5 mg/Kg) was ineffective in this process. It was seen that the animals stimulated with MTA (50 mg/cavity) showed a significative amount of IL-1ß and MIP-2 released to the peritoneal exudate. The pretreatment with Thioglycolate 3% increased 380% the macropahge population into the peritoneal cavity, increasing the MTA-induced neutrophil migration (p<0.05). The pretreatment with 48/80 compound decreased 75% the mast cell population in the peritoneal cavity and decreased the MTAinduced neutrophil migration (p<0.05). The injection of MTA in the air-pouch cavity induced a neutrophil migration, however, the recruitment was shorter than that induced into the peritoneal cavity. These data confirm the participation of the mast cell and macrophages in the MTA-induced neutrophil migration. The injection of MTA-stimulated macrophages and mast cells supernatants into the mice peritoneal cavity induced a significant neutrophil migration that was inhibited by the pretreatment with dexamethasone (10 æMolar), BWA4C (100 æMolar) and U75302 (10 æMolar) suggesting the release of LTB4 and cytokines and/or chemokines by these cells. Besides, macrophages and mast cells MTA-induced were able to express in vitro IL-1ß MIP-2 and 5-LO mRNA. In conclusion, the neutrophil migration into mice peritoneal cavity induced by MTA was dependent on mast cells and macrophages, which expressed IL-1ß, MIP-2 and LTB4.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.unesp.br:11449/95476 |
| Date | 21 December 2006 |
| Creators | Gomes, Alessandra Cristina [UNESP] |
| Contributors | Universidade Estadual Paulista (UNESP), Oliveira, Sandra Helena Penha de [UNESP] |
| Publisher | Universidade Estadual Paulista (UNESP) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | 118 f. : il. + 1 CD-ROM |
| Source | Aleph, reponame:Repositório Institucional da UNESP, instname:Universidade Estadual Paulista, instacron:UNESP |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -1, -1 |
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