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Previous issue date: 2012-05-15 / Objectives: To conduct a preliminary analysis of the therapeutic effects of
laser therapy (830nm) with regard to surface topography (scanning electron
microscopy - SEM) and chemical composition (energy dispersive spectrometry -
EDS) of the newly formed bone during distraction osteogenesis (DO) combined with
low-level laser therapy (LLLT) in an experimental sheep model.
Methods: Five female Corriedale sheep weighing 40 to 45kg (experimental
units A, B, C, D, and E) were treated with LLLT at different stages of OD, as follows:
sheep A, B, and C received the first irradiation session immediately after closure of
the surgical wound and new sessions then every 48 hours (at a total of eight
sessions); sheep D received the first irradiation session on the last day of device
activation, followed by seven additional applications every 48 hours (at a total of eight
sessions); sheep E (control) was not irradiated. Bone samples were analyzed via
SEM at 80x, 300x, and 1000x magnification, and EDS spectra, at 300x magnification.
Results: According to the Student t test for paired samples, the medullary
bone of sheep mandibles treated with DO presented statistically higher mean values
of carbon and calcium when compared with untreated mandibles (controls). The
internal and external cortical layers did not show statistically significant differences in
relation to reference values (p > 0.05). Bone surface topography was very similar in
all specimens.
Conclusion: Infrared laser therapy as used in our study (GaAlAs, 830nm,
50mW power, cutaneous irradiation at three sites, and energy density of 5J/cm2
per
session at a total of 120J/cm2), has benefits when applied at the beginning of the
bone consolidation period, suggesting an increased hardness and resistance of the
newly formed bone tissue / Objetivos: Verificar, em estudo preliminar, a import?ncia da utiliza??o
terap?utica da laserterapia (830nm) no que diz respeito ? topografia ?ssea
(Microscopia Eletr?nica de Varredura - MEV) e a composi??o qu?mica
(Espectr?metro por Dispers?o de Energia - EDS) do osso neoformado mandibular
por distra??o osteog?nica (DO) associada ? laserterapia (LLLT) em modelo
experimental animal ovelha.
Metodologia: A amostra deste estudo foi composta por cinco ovelhas f?meas
da ra?a Corriedale, com peso entre 40 e 45 quilos, divididas em cinco unidades
experimentais (grupos A, B, C, D e E), com utiliza??o do laser em diferentes etapas
da distra??o osteog?nica. Nos grupos A, B e C, a irradia??o inicial foi aplicada logo
ap?s o t?rmino da sutura e a cada 48 horas (total de oito aplica??es). No grupo D, a
primeira irradia??o ocorreu no ?ltimo dia da ativa??o do distrator, seguindo com mais
sete aplica??es a cada 48 horas (totalizando de oito aplica??es). O grupo E
(controle) n?o recebeu nenhuma irradia??o. Os pontos foram analisados no MEV
com magnifica??o de 80x, 300x e 1000x e os espectros por EDS realizados sobre a
imagem em um aumento de 300X.
Resultados: De acordo com o teste t-Student pareado, na medular das
ovelhas que sofreram distra??o ?ssea, os valores m?dios de Carbono e C?lcio s?o
estatisticamente maiores que as respectivas m?dias. Nas corticais interna e externa
n?o ocorreram diferen?as estatisticamente significativas nas m?dias dos elementos
comparados aos valores de refer?ncia (p > 0,05). A topografia ?ssea mostrou-se
muito semelhante em todas as amostras.
Conclus?o: A laserterapia com comprimento de onda infravermelha (GaAlAs,
830nm), 50mW de pot?ncia, irradia??o cut?nea com tr?s pontos de densidade de
energia de 5J/cm2
por sess?o, totalizando 120J/cm2
ao final do tratamento, ?
ben?fica quando aplicada no per?odo de matura??o do osso, pois sugere um
aumento na dureza do tecido ?sseo neoformado
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1178 |
| Date | 15 May 2012 |
| Creators | Blaya, Diego Segatto |
| Contributors | Oliveira, Mar?lia Gerhardt de |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, BR, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 500, 600, 4673435736271820140 |
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