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Previous issue date: 2014-02-24 / Imbalance in hemostatic mechanisms can occur during pregnancy with a tendency for hypercoagulability and increased thrombosis risk. Pregnant women with a hypertensive disorder, especially preeclampsia, may disclose alterations in total platelet count and the platelet indexes, mean platelet volume (MPV) and immature platelet fraction (IPF). The IPF has been suggested as a sensitive index for monitoring changes in platelet production and destruction. An automated technique for obtaining this parameter has been used. The aim of the present study was to evaluate the immature platelet fraction behavior in patients diagnosed with a gestational hypertensive disorder (GHD), using fluorescent flow cytometry. A crosssectional study conducted at the S?o Lucas Hospital, Porto Alegre, Brazil to estimate IPF levels in the maternal blood of 99 pregnant women, divided into 3 groups: normotensive controls (NP), preeclampsia syndrome (PES) and non-proteinuric hypertensive pregnancy (nPHP). Following ethical approval and written informed consent, samples were collected from 33 NP, 34 PES, and 32 nPHP women. IPF levels were measured by fluorescent flow cytometry using the XE-5000? (Sysmex Corporation, Kobe, Japan). The IPF and MPV count in pregnant women with a GHD was significantly higher than the control group. No significant difference was detected between the PES and nPHP groups. The MPV for the NP group was normal. A distinct profile in platelet indexes was detected in hypertensive pregnancies. It is suggested that these markers could be used in daily routine as an additional tool in the management of hypertensive pregnant women. / Um desequil?brio nos mecanismos hemost?ticos pode ocorrer durante a gesta??o com tend?ncia ? hipercoagulabilidade e aumento do risco de trombose. Nas gestantes com dist?rbio hipertensivo, principalmente nas pr?-ecl?mpticas, h? dados na literatura que indicam haver altera??o no n?mero total de plaquetas e nos ?ndices plaquet?rios, volume plaquet?rio m?dio (VPM) e fra??o de plaquetas imaturas(IPF). A IPF tem sido sugerida como um ?ndice sens?vel para monitorar mudan?as na produ??o e destrui??o plaquet?ria. Uma t?cnica automatizada para a obten??o deste par?metro tem sido utilizada. O objetivo do presente estudo ? avaliar o comportamento da IPF utilizando citometria de fluxo fluorescente em pacientes com diagn?stico de doen?a hipertensiva gestacional. Foi realizado estudo transversal controlado com 99 gestantes a fim de estimar os n?veis de IPF no sangue materno das pacientes com s?ndrome de pr?-ecl?mpsia (SPE) comparando com hipertensas gestacionais sem protein?ria (HGsP) e gestantes normais normotensas (NT) atendidas no Hospital S?o Lucas/PUCRS, Porto Alegre, Brasil. O termo de consentimento livre e esclarecido foi empregado nas 34 gestantes com SPE, 32 com GHsP e nas 33 NT. A IPF foi contada atrav?s do equipamento XE-5000 (Sysmex Corporation, Kobe, Japan), utilizando citometria de fluxo fluorescente. A contagem de IPF e VPM em gestantes com doen?a hipertensiva gestacional (DHG) foi significativamente maior que no grupo controle. N?o houve diferen?a estat?stica entre os grupos SPE e HGsP. O VPM foi normal em todas as gestantes do grupo controle. Detectou-se um distinto perfil dos ?ndices plaquet?rios em gestantes hipertensas, e sugere-se que esses marcadores possam ser utilizados na rotina cl?nica di?ria como uma ferramenta adicional no aux?lio do diagn?stico precoce da DHG.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1774 |
| Date | 24 February 2014 |
| Creators | Moraes, Daniela |
| Contributors | Figueiredo, Carlos Eduardo Poli de |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de, PUCRS, BR, Faculdade de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7620745074616285884, 500, 600, -8624664729441623247 |
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