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Previous issue date: 2014-02-12 / Hepatocellular carcinoma (HCC) is the most frequent primary tumor of the liver, and is currently one of the major neoplastic diseases, representing 85% of primary liver tumors and is the third leading cause of cancer-related death. The development of the majority of hepatocellular carcinomas related to the presence of a disease resulting from cirrhosis, such as hepatitis B and C. Its incidence has increased in recent years due to the number of patients with infections caused by hepatitis C. The aim of this study was investigate the in vitro effect of fructose-1,6-bisphosphate (FBP) on the growth of HepG2 cells, used as a model to hepatocellular carcinoma. The results showed that the FBP decreases cell proliferation in a dose-dependent manner, not being caused by cell cycle arrest or apoptosis verified by flow cytometry. It was observed that at 72 h of treatment FBP decreased levels of IL-8, which is closely related to the tumor progression and the generation of reactive oxygen species. Moreover, FBP decreased oxidative stress by increasing the levels of catalase and decrease TBARS, this effect is possibly caused by the result of low IL- 8. These findings demonstrated that the FBP may be a potential anticancer agent for the treatment of HCC. / O hepatocarcinoma (HCC) ? o tumor prim?rio de f?gado mais frequente, sendo atualmente uma das principais doen?as neopl?sicas, representando 85% dos tumores hep?ticos prim?rios e sendo a terceira maior causa de morte relacionada ao c?ncer. O desenvolvimento da maioria dos hepatocarcinomas relaciona-se com a presen?a de alguma doen?a decorrente do quadro de cirrose, como hepatite B e C. Sua incid?ncia tem aumentado nos ?ltimos anos devido ao n?mero de pacientes com infec??es por hepatite C. O objetivo deste estudo foi investigar,in vitro, o efeito da frutose-1,6-bisfosfato (FBP) sobre o crescimento das c?lulas HepG2, modelo utilizado como carcinoma hepatocelular. Os resultados demonstraram que a FBP diminui a prolifera??o celular em uma rela??o dose-dependente, n?o sendo essa prolifera??o ocasionada por parada no ciclo celular ou apoptose verificado pelos testes de citometria. Observou-se que em 72 h de tratamento a FBP diminuiu os n?veis de Interleucina 8 (IL-8), sendo esta interleucina relacionada com a progress?o tumoral e a gera??o de esp?cies reativas de oxig?nio. Al?m disso, a FBP diminuiu o estresse oxidativo, aumentando os n?veis de catalase e diminuindoa produ??o das subst?ncias reativas ao ?cido tiobarbit?rico(TBARS), sendo esse efeito antioxidante possivelmente ocasionado por conseq??nte diminui??o de IL-8. Estes resultados demonstraram que a FBP pode ser um agente antineopl?sico em potencial para o tratamento de hepatocarcinoma.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/5499 |
| Date | 12 February 2014 |
| Creators | Krause, Gabriele Catyana |
| Contributors | Oliveira, Jarbas Rodrigues de |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Biologia Celular e Molecular, PUCRS, BR, Faculdade de Bioci?ncias |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 8198246930096637360, 600, 600, 36528317262667714 |
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