Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-06-11T19:45:55Z
No. of bitstreams: 1
470271 - Texto Completo.pdf: 601264 bytes, checksum: 631a8bec7a15cd2f97fae4e0bb07768a (MD5) / Made available in DSpace on 2015-06-11T19:45:55Z (GMT). No. of bitstreams: 1
470271 - Texto Completo.pdf: 601264 bytes, checksum: 631a8bec7a15cd2f97fae4e0bb07768a (MD5)
Previous issue date: 2015-03-24 / The esophageal and oral tumors are classified as the most frequent malignancies in Brazil. Esophageal cancer is the eighth most common in the world and oral cancer is ranked as the 5th among malignant neoplasms affecting man in Brazil. The lipopolysaccharide (LPS) are characteristic compounds of the cell wall of gram-negative bacteria. They are able to regulate gene expression of pro-inflammatory cytokines by binding to toll-like receptor 4 (TLR4) via NF-kB pathway. Recent studies show that LPS can increase the migration ability of cell lines of human esophageal cancer HKESC-2 by increasing its binding properties. In the meantime, it has not been tested the effect of LPS on esophageal cancer cells OE19 and OE21 and human oral carcinoma HN30. Thus, this study aimed to determine the action of LPS compounds on the proliferation and viability of cell lines of human esophageal cancer and human oral carcinoma HN30. Were used as treatment LPS for OE19 and OE21 cell lines and the PgLPS (lipopolysaccharide of Porphyromonas gingivalis) for HN30 cell line. Cell viability was assessed using the MTT assay and cell counting. The TLR4 expression by real-time PCR was also evaluated. LPS at higher concentrations decreased significantly cell viability in both cell lines, adenocarcinoma (OE19) and squamous esophageal carcinoma (OE21) at different times of treatment. In addition, both cell lines, OE19 and OE21, expressed TLR4 receptor. Taken together, our data demonstrated that LPS at high concentrations might contribute to tumor death, in agreement with previously data. / Os tumores de es?fago e de boca est?o classificados como as neoplasias malignas mais frequentes no Brasil. O c?ncer de es?fago ? o oitavo mais comum no mundo e o c?ncer de boca ? classificado como o 5? dentre as neoplasias malignas que afetam o homem no Brasil. Os lipopolissac?ridos (LPS) s?o compostos caracter?sticos da parede celular de bact?rias gram-negativas. Eles s?o capazes de regular a express?o de genes de citocinas pr?-inflamat?rias, atrav?s da liga??o ao receptor toll-like 4 (TLR4) via NF-kB. Estudos recentes mostram que o LPS pode aumentar a habilidade de migra??o de linhagens c?lulares de c?ncer de es?fago humano HKESC-2 atrav?s do aumento de suas propriedades de ades?o. Entretanto, ainda n?o foi testado o efeito do LPS sobre as c?lulas de c?ncer de es?fago e de carcinoma oral humano HN30. Deste modo, este estudo teve como objetivo determinar a a??o dos compostos de LPS (derivado de bact?rias) sobre a prolifera??o e viabilidade de linhagens celulares de c?ncer de es?fago humano, e de carcinoma oral humano. Foram utilizados como tratamento o LPS para as linhagens OE19 e OE21 e o PgLPS (lipopolissacar?deo da Porphyromonas gingivalis) para a linhagem HN30. A viabiliadade celular foi avaliada utilizando o ensaio MTT e contagem celular. Tamb?m foi avaliada a express?o do receptor TLR4 por PCR em tempo real. LPS em concentra??es mais elevadas reduziu significativamente a viabilidade celular em ambas as linhagens celulares de c?ncer de es?fago, o adenocarcinoma (OE19) e o carcinoma de c?lulas escamosas (OE21) em diferentes tempos de tratamento. Al?m disso, ambas as linhagens celulares, OE19 e OE21, expressaram o receptor TLR4. Avaliados em conjunto, os nossos dados demonstram que o LPS em concentra??es elevadas pode contribuir para a morte tumoral, de acordo com dados pr?vios.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6125 |
| Date | 24 March 2015 |
| Creators | Gon?alves, M?rcia |
| Contributors | Morrone, Fernanda Bueno |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Biotecnologia Farmac?utica, PUCRS, Brasil, Faculdade de Farm?cia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2304961219518893267, 600, 600, 600, -8380654636843378116, 6997636413449754996 |
Page generated in 0.0848 seconds