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Previous issue date: 2015-10-15 / The aim of this study was to evaluate dental pulp and furcal periodontal tissue
responses to mineral trioxide aggregate (MTA) and to a calcium silicate-based
material (Biodentine?). The lower first molars in hundred forty (140) male Wistar rats
were divided into groups according to the experimental site and period (14 and 21
days) testing two materials (n=8 per group/period in pulpotomy and n=6 per
group/period in furcal perforation). Groups capped and sealed with MTA or
Biodentine? were restored with silver amalgam; in an additional test group, teeth
were capped and restored with Biodentine?. Teeth capped with gutta-percha and
restored with silver amalgam served as a positive control and untouched teeth were
negative control. Histological evaluation of pulp responses and macroscopic
examination of coronal sealing were performed. Inflammation extension and intensity,
degenerative events and formation of mineralized tissue barrier were assessed. In
the experiment of furcal perforations, inflammation extension and intensity, bone
resorption and cement formation were assessed histologically. All results underwent
ANOVA and Tukey post hoc tests (p<.05). In pulpotomy, Biodentine? and MTA
presented satisfactory results, which were characterized by a mild inflammatory
response (p<.0001) and more pronounced formation of mineralized barrier (p<.0001)
compared to teeth capped with gutta-percha. When used as a restorative material,
Biodentine? kept coronal sealing in only 37.5% of the samples. As to furcal
perforations, Biodentine? and MTA presented satisfactory results, which were
characterized by a milder inflammatory response compared to the control, regardless
of the material used for coronal sealing and of the experimental period evaluated
(p<.0001). All test groups showed less bone resorption than the positive control after
21 days (p<.05), being this difference more pronounced in teeth restored with silver
amalgam. Cement repair occurred in 30% of MTA and Biodentine? samples, and
was not detected in any specimen of the positive control group. In conclusion,
Biodentine? and MTA promoted similar responses when used to pulp capping and
furcal perforation sealing. On the other hand, the use of Biodentine? as temporary
restorative material did not promote efficient coronal sealing. Although further
investigations are required, Biodentine? can be considered an alternative for
pulpotomy and furcal perfuration. / O objetivo deste estudo foi comparar a resposta tecidual, ap?s pulpotomias e
perfura??es de furca, utilizando agregado tri?xido de mineral (MTA) e o material ?
base de silicato tric?lcico (Biodentine?). Os primeiros molares inferiores de 140 ratos
Wistar machos foram utilizados em dois tempos (14 e 21 dias) e divididos em grupos
conforme o experimento e materiais utilizados (pulpotomia n=8 e perfura??o de furca
n=6). Nos dois experimentos, grupos capeados e selados com MTA e Biodentine?
foram restaurados com am?lgama de prata; um grupo adicional utilizou somente
Biodentine? e o controle positivo recebeu guta-percha e restaura??o de am?lgama;
dentes intactos serviram de controle negativo. No experimento de pulpotomias,
realizou-se exame macrosc?pico e radiogr?fico do selamento coron?rio e exame
histol?gico dos eventos inflamat?rios (intensidade e extens?o), degenerativos e de
forma??o de barreira mineralizada. No experimento de perfura??es de furca, foram
avaliados histologicamente os eventos inflamat?rios (intensidade e extens?o), de
reabsor??o ?ssea e reparo de cemento. Os dados foram comparados atrav?s da
an?lise de vari?ncia (ANOVA) e posthoc de Tukey (p<.05). Nas pulpotomias, o
Biodentine? e MTA apresentaram resultados satisfat?rios, com menor resposta
inflamat?ria (p<.0001) e pronunciada forma??o de barreira mineralizada (p<.0001)
comparada aos dentes do controle positivo. O Biodentine?, utilizado como
restaura??o, manteve o selamento coron?rio em apenas 37.5% das amostras. Nas
perfura??es de furca, o Biodentine e MTA apresentaram resultados satisfat?rios,
caracterizados por uma resposta inflamat?ria mais suave em compara??o ao
controle positivo, independentemente do material utilizado para o selamento
coron?rio e per?odo experimental avaliado (p<.0001). Todos os grupos testes
mostraram menor reabsor??o ?ssea do que o controle positivo ap?s 21 dias (p<.05),
sendo esta diferen?a mais acentuada em dentes restaurados com am?lgama de
prata. A repara??o de cemento ocorreu em 30% das amostras de MTA e Biodentine,
e n?o foi detectada em qualquer amostra do grupo do controle positivo. Concluiu-se
que houve respostas similares ap?s o uso de Biodentine? e MTA no capeamento da
polpa exposta e selamento de perfura??es de furca. Por outro lado, o uso do
Biodentine? como material restaurador provis?rio n?o promoveu selamento coron?rio
eficiente. Embora sejam necess?rias novas investiga??es, o Biodentine deve ser
considerado como uma alternativa frente aos tratamentos propostos.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6502 |
| Date | 15 October 2015 |
| Creators | Reis, Magda de Sousa |
| Contributors | Figueiredo, Jos? Antonio Poli de, Scarparo, Roberta Kochenborger |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, Brasil, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 600, 600, 600, 4673435736271820140, -2070498469879244349 |
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