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Previous issue date: 2017-02-15 / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / The Phylum Nematoda is extremely diverse and occurs in several ecosystems, thus occupying a key role in the soil trophic webs. The knowledge of nematode species and their patterns of distribution in soils habitats may contribute to our understanding of soil ecology and functioning. The aims of this work was to describe the nematofauna occurring in the Center of Research and Environmental Conservation - PR?-MATA-RS and classifies the nematodes according to their feeding habits thus provide additional information about the Pr? Mata soil diversity. Identification was performed through light microscopy and following taxonomic keys evidencing the diagnostic characters of each group. In total, 1.500 nematodes were found into 200 cm? of soil analyzed. The Chromadorea class was represented by 63% of the individuals, whereas the class Enoplea class by only 12%. Four orders, eight families and 13 genera were recovered. As for feeding trophic groups, were represented plant parasites by 35%, bacterial feeders by 30%, omnivores by 7% and predators by 5%. After extracting nematodes from soil, DNA extraction for amplification of the V4 and V9 regions of the 18S gene of ribosomal DNA was performed. The amplified product was sequenced, obtaining 281,400 sequences passed. Based on the DNA sequences, seven orders, 15 families, and 16 nematode genera were recovered. / O filo Nematoda ? bastante diverso e ocorre em v?rios ecossistemas, ocupando uma posi??o central na cadeia tr?fica de solos. O conhecimento das esp?cies e distribui??o destas pode contribuir em estudos sobre a ecologia e funcionamento do solo, incluindo a din?mica de popula??o e controle biol?gico n?o s? dos pr?prios nemat?deos, mas tamb?m do ecossistema ali presente. Este trabalho teve como objetivo descrever a nematofauna ocorrente na ?rea do Centro de Pesquisas e Conserva??o da Natureza PR?-MATA-RS, e classific?-la taxonomicamente e de acordo com seu grupo tr?fico. A identifica??o foi realizada atrav?s de microscopia ?ptica baseada em chaves taxon?micas evidenciando os caracteres diagn?sticos de cada grupo. Mil e quinhentos nemat?deos foram encontrados em 200 cm? de solo. 63% pertencentes ? classe Chromadoria e 12% ? classe Enoplea, classificados em quatro ordens, oito fam?lias e 13 g?neros. Quanto aos h?bitos alimentares dos nemat?deos foram classificados como 35% parasitos de plantas, 28% bacter?voros, 7% on?voros e 5% como predadores. Ap?s isolamento dos nemat?deos, foi realizada extra??o de DNA para a amplifica??o das regi?es V4 e V9 do gene 18S do DNA (rDNA). O produto amplificado foi sequenciado, obtendo-se 281.400 sequ?ncias. Dentre o filo Nematoda, as fam?lias classificadas foram: Cephalobidae, Chromadoridae, Criconematidae, Plectidae, Ironidae, Prismatolaimidae, Rhabdolaimidae e Tripylidae.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/7686 |
| Date | 15 February 2017 |
| Creators | M?ller, Carla Aristonara |
| Contributors | Graeff-Teixeira, Carlos |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Zoologia, PUCRS, Brasil, Faculdade de Bioci?ncias |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2008925231902741151, 600, 600, 600, 600, 36528317262667714, -6482652380601267558, -2555911436985713659 |
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