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Previous issue date: 2015-07-24 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The powdery mildew is one of the most important foliar diseases that attack the watermelon and other cucurbits in Brazil and in the world. The principal identification elements by the consumer are based on the external appearance and quality of the fruit. The objective of this study was to identify microsatellite markers linked to resistance to powdery mildew and to the stripe pattern of watermelon fruits with microsatellite markers. Plants of the parents, F1 and F2, which are the result of a cross between the cultuvars BRS Opara (powdery mildew resistant and with clearly defined stripes fruit) and P?rola (powdery mildew susceptible and with diffuse stripes fruit), were phenotyped in the field for resistance or susceptibility to powdery mildew and, after of the harvest, the fruits was morphologically characterized. For these characteristics, 116 microsatellite markers were analyzed and the linkage analysis done in JoinMap 2.0. Segregation in the F2 population demonstrated that resistance powdery mildew and the stripe pattern are controlled by a single dominant gene. The microsatellite loci MCPI_11, CYSTSIN and BVWS02441 showed linked to the powdery mildew resistance gene at 2.6 cM with LODs ?score? of 31.42 and are located in chromosome two of the watermelon genome. The microsatellite loci MCPI_05 and MCPI_16 showed a linkage to the stripe patterns of watermelon fruits at a distance of 1.5 and 1.8 cM with a LODs ?scores? of 39.28 and 38.11 respectively, and are located in the chromosome six of the watermelon genome. These markers can be used in the marker assisted selection process in watermelon improvement programs. / O o?dio ? uma das principais doen?as foliares que acomete a melancia e outras cucurbit?ceas, no Brasil e no mundo. Os principais elementos de identifica??o de uma cultivar pelo consumidor est?o relacionados com a apar?ncia externa e qualidade do fruto. O objetivo deste trabalho foi identificar marcadores microssat?lites ligados ? resist?ncia ao o?dio e ao padr?o de listras de frutos de melancia. Plantas dos parentais, de F1 e de F2, resultantes do cruzamento entre as cultivares BRS Opara (resistente ao o?dio e com padr?o de listras claramente definidas) e P?rola (suscet?vel ao o?dio e com padr?o de listras difusas) foram fenotipadas em campo para resist?ncia ou suscetibilidade ao o?dio e, ap?s a colheita, os frutos foram caracterizados quanto ao padr?o de listras. Para essas duas caracter?sticas foram analisados 116 marcadores microssat?lites e as an?lises de liga??o foram realizadas no programa JoinMap 2.0. A segrega??o da popula??o F2 demonstrou que a resist?ncia ao o?dio e o padr?o de listras de frutos de melancia s?o caracter?sticas monog?ncias e dominantes. Os microssat?lites MCPI_11, CYSTSIN e BVWS02441 mostraram-se ligados ao gene que confere resist?ncia ao o?dio a 2,6 cM com LODs ?score? de 31,42 e est?o localizados no cromossomo dois no genoma da melancia. Os microssat?lites MCPI_05 e o MCPI_16 est?o ligados ao padr?o de listras de frutos de melancia a uma dist?ncia de 1,5 e 1,8 cM com LODs ?score?de 39,28 e 38,11, respectivamente, e est?o localizados no cromossomo seis do genoma da melancia. Esses marcadores poder?o ser utilizados no processo de sele??o assistida por marcadores em programas de melhoramento de melancia.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.uefs.br:8080:tede/233 |
| Date | 24 July 2015 |
| Creators | Gama, Renata Nat?lia C?ndido de Souza |
| Contributors | Santos, Carlos Antonio Fernandes |
| Publisher | Universidade Estadual de Feira de Santana, Doutorado Acad?mico em Recursos Gen?ticos Vegetais, UEFS, Brasil, DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UEFS, instname:Universidade Estadual de Feira de Santana, instacron:UEFS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2074743607827725103, 600, 600, 600, 600, -6971480722008537872, -1634559385931244697, 2075167498588264571 |
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