A long-range research interest of this laboratory is the evolution of the transcriptional control mechanisms of the vertebrate troponin I (TnI) gene family. It is likely that the vertebrate TnI gene family arose from a single TnI gene present in early chordate ancestors. Analysis of transcriptional control mechanisms of the TnI gene of a primitive chordate, such as the ascidian Ciona intestinalis, may therefore provide insight into the regulatory mechanisms of the vertebrate ancestral TnI gene. As an initial step in such a study, I localized transcriptional control regions within 1.5 kb of 5'-flanking DNA of the Ciona TnI gene. I prepared a series of deletion constructs in which Ciona TnI 5'-flanking DNA segments were fused to a nuclear-targeted beta-galactosidase reporter gene. Constructs were introduced into fertilized Ciona eggs by electroporation, and following development up to the mid tailbud stage (12 h), reporter gene expression was assessed by whole-mount beta-galactosidase histochemistry. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.33733 |
| Date | January 2002 |
| Creators | Cleto, Cynthia. |
| Contributors | Hastings, Ken (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001872159, proquestno: MQ78850, Theses scanned by UMI/ProQuest. |
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