Interactions between serum lipoproteins and human cells in the secretion of glycosaminoglycans (GAG) were investigated in cultures of arterial smooth muscle cells (SMC) and fibroblasts. Lipoprotein free serum (LFS) supported cell proliferation but caused a dose-dependent decrease in GAG secretion and cell cholesterol. Addition of low density lipoproteins (LDL) to 10% LFS in the medium caused increases in GAG secretion and cell cholesterol but a net decrease in cell population. LDL effects were positively correlated with the rate of cell proliferation independent of serum concentration. High density lipoproteins (HDL) decreased GAG secretion in slowly but not in actively proliferating cultures; however in the latter, it reduced all LDL-induced changes. Cells from non insulin-dependent diabetics had an increased GAG response to LDL. Low concentrations of platelet factors enhanced GAG secretion. The addition of a GAG mixture inhibited all LDL effects. On the basis of these observations, a hypothetical model is presented indicating that GAGs play a significant role in the development of atherosclerosis.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.71853 |
| Date | January 1982 |
| Creators | Wosu, Leonard O. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000186819, proquestno: AAINK64626, Theses scanned by UMI/ProQuest. |
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