The endocannabinoid (EC) system has a role in metabolic homeostasis. The purpose
of this study was to determine the effect of ECs and the fatty acids they are derived
from on lipid metabolism and mitochondrial function in adipocytes. 3T3-L1
adipocytes on day 8 of differentiation were treated with ECs and fatty acids for 48
hours in the absence or presence of insulin and various inhibitors. Lysates were
analyzed via Western immunoblotting, a lipolysis assay and Seahorse XF Analyzer
for changes in protein levels, phosphorylation state, lipolysis, and oxygen
consumption rate. Results showed that ECs (2-arachidonoyl glycerol) stimulated
lipolysis via a novel AMPK-dependent pathway, while fatty acids had varying effects
on insulin signaling and mitochondrial function . These data suggest adipose tissue
EC receptors may be a suitable target for anti-obesity therapy. Further research is
needed to understand how the dietary fatty acid profile may influence synthesis of
ECs. / May 2016
| Identifer | oai:union.ndltd.org:MANITOBA/oai:mspace.lib.umanitoba.ca:1993/31203 |
| Date | 12 April 2016 |
| Creators | Siemens, Linda |
| Contributors | Taylor, Carla (Human Nutritional Sciences) Zahradka, Peter (Physiology and Pathophysiology), Suh, Miyoung (Human Nutritional Sciences) Fernyhough, Paul (Pharmacology and Therapuetics) |
| Source Sets | University of Manitoba Canada |
| Detected Language | English |
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