The auxins, including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA), promotes the formation of adventitious root in hypocotyls of soybean (Glycine max). IBA significantly promotes the formation of adventitious roots more than IAA and NAA . The activity of anionic pI 3.7 peroxidase (POX) and cationic pI 8.5 POX were inhibited by exogenous auxins during the induction of adventitious root on day 2. Besides, the activity of pI 5.3 POX was enhanced by IBA during the initiation stage on day 4. The increase of the activity of pI 5.3 POX was accompanied by the increase of H2O2 levels. In the previous researches, it shows that the promoter of pI 8.5 POX gene contains both ARF/AuxRE and CATATGGMSAUR motifs that are responded to auxins. In this studies, the pI 5.3 POX gene, which responses to auxins a day or two later, contains only ARF/AuxRE motif. The regulation of pI 5.3 POX gene is probably initiation phase-dependent. The results suggest that anionic pI 5.3 POX produces significant amount of H2O2 through the binding of auxin to POX and mediate the auxin signaling pathway leading to plant growth .
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0209109-101810 |
| Date | 09 February 2009 |
| Creators | Huang, Yi-chi |
| Contributors | Ching-mei Hsu, Zin-huang Liu, Mang-jye Ger |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0209109-101810 |
| Rights | restricted, Copyright information available at source archive |
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