Abstract
Nervous necrosis virus of Epinephelus malabaricus (MGNNV) belongs to the genus of Piscinodavirus (Betanodavirus) that causes vacuolating encephalopathy and retinopathy or viral nervous necrosis. MGNNV propagated in SSN-1 cells that were derived from fry tissue of striped snakehead fish, Channa striatus. SSN-1 was highly permissive to MGNNV infection and production, in which the TCID50 per ml increased from the
magnitude of 10 4 to 10 8 after 5 passages. The thermal stability analysis unveiled that the optimal activity of MGNNV can be resumed at the
temperature from frozen to less than 25 ¢J. Oreochromis mossambica was highly susceptible to MGNNV only when injecting the virus into the vitreous body of fish eye, via which the virus could propagate in the fish brain. Employing the technique of immobilizing virus on itrocellulose, several cellular MGNNV-binding proteins were detected. Two MGNNV-binding proteins of 100 and 56 kDa were found in SSN-1 cell lysate; four proteins of 60, 43, 36, and 30 kDa in rotifer lysate; four proteins of 62, 50, 20 and 25 kDa
on the membrane of grouper fertilized eggs. In different organ lysates of Epinephelus malabaricus, two MGNNV-binding proteins of 80 and 43 kDa were found in brain; four MGNNV-binding proteins of 60, 45, 43 and 23 kDa were found in eye; two MGNNV-binding proteins of 80, 18 kDa were found in liver; four MGNNV-binding proteins of 60, 45, 43 and 30 kDa were found in pancreas; two MGNNV-binding proteins of 45 and 24 kDa were found in spleen; one MGNNV-binding proteins of 80 kDa were found in kidney; six MGNNV-binding proteins of 100, 95, 80, 48, 45 and 30 kDa were found in ovary.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0719101-150136 |
| Date | 19 July 2001 |
| Creators | Lin, Chun-Ju |
| Contributors | Chi-Hsin Hsu, Chan-Shing Lin, Jong-Kang Liu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0719101-150136 |
| Rights | not_available, Copyright information available at source archive |
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