Global ETD Search

1	Dectection of binding prteins of Epinephilus malabaricus nervous necrosis virus Lin, Chun-Ju 19 July 2001 (has links) Abstract Nervous necrosis virus of Epinephelus malabaricus (MGNNV) belongs to the genus of Piscinodavirus (Betanodavirus) that causes vacuolating encephalopathy and retinopathy or viral nervous necrosis. MGNNV propagated in SSN-1 cells that were derived from fry tissue of striped snakehead fish, Channa striatus. SSN-1 was highly permissive to MGNNV infection and production, in which the TCID50 per ml increased from the magnitude of 10 4 to 10 8 after 5 passages. The thermal stability analysis unveiled that the optimal activity of MGNNV can be resumed at the temperature from frozen to less than 25 ¢J. Oreochromis mossambica was highly susceptible to MGNNV only when injecting the virus into the vitreous body of fish eye, via which the virus could propagate in the fish brain. Employing the technique of immobilizing virus on itrocellulose, several cellular MGNNV-binding proteins were detected. Two MGNNV-binding proteins of 100 and 56 kDa were found in SSN-1 cell lysate; four proteins of 60, 43, 36, and 30 kDa in rotifer lysate; four proteins of 62, 50, 20 and 25 kDa on the membrane of grouper fertilized eggs. In different organ lysates of Epinephelus malabaricus, two MGNNV-binding proteins of 80 and 43 kDa were found in brain; four MGNNV-binding proteins of 60, 45, 43 and 23 kDa were found in eye; two MGNNV-binding proteins of 80, 18 kDa were found in liver; four MGNNV-binding proteins of 60, 45, 43 and 30 kDa were found in pancreas; two MGNNV-binding proteins of 45 and 24 kDa were found in spleen; one MGNNV-binding proteins of 80 kDa were found in kidney; six MGNNV-binding proteins of 100, 95, 80, 48, 45 and 30 kDa were found in ovary. Betanodavirus Piscinodavirus NNV MGNNV binding protein
2	The Studies on assembly of Dragon Grouper Nervous Necrosis Virus and virus-like particles Wu, Yi-min 26 August 2008 (has links) Piscine nodaviruses are members of genus Betanodavirus, which infect more than 30 species of fish and cause massive mortality in larvae and juveniles. The infection causes great economic losses to aquaculture and sea-ranching. To study the dissociation and reassembly of betanodavirus, virus-like particles (VLPs) of dragon grouper nervous necrosis virus (DGNNV) were used. The experiments with calcium-chelating or reducing/oxidizing reagents elicited that the DGNNV VLPs required only calcium for particle assembly. With the recombinant VLPs, site-directed mutagenesis can be employed to investigate the roles of calcium-binding ligands in particle formation. In the mutational analysis of DxxDxD that is putatively involved in the coordination of calcium ions, the results showed that the D133N mutation significantly disrupted the assembly of VLPs while D130N and D135N mutants produced heterogeneous particles with broken shapes. The thermal stability of the VLP-forming fractions demonstrated that VLPs of D135N mutant were stable at a temperature of 85¢XC, which is slightly higher than that for wild-type, whereas VLPs of D130N mutant could not tolerate the thermal effects at a temperature higher than 60¢XC. It is deduced that three aspartate residues of the motif DxxDxD are all important for the efficient formation of DGNNV VLPs and, among them, the DxxD provides a more stable coordinate of calcium-ligand than DxD. Virus-like particles NNV Dragon grouper
3	Åtgärder för ökad markanvändning i solcellspark : En tekno-ekonomisk fallstudie om potentialen hos bifacial och solföljare i Solpark Fyrislund Majid, Safwat January 2021 (has links) Solar parks are increasingly getting a larger market share of PV installations over the world, and have for the last couple of years managed to establish itself in the Swedish market. The market has for a long time been known for its decline in module prices, which has allowed an emergence of more efficient PV-techniques such as one-axis trackers and bifacial modules. Bifacial modules use the backside of modules for improved utiliziation of incoming light, while one-axis trackers have the ability to track the sun in order to maximise light absorption. These innovations have now caught the interest of companies willing to invest in large-scale PV-farms, where efficient land use is highly regarded. The aim of this thesis was to examine how bifacial modules and one-axis trackers perform in terms of system performance and profitabilty if implemented in 'Solpark Fyrislund', a solar park owned by Vasakronan AB. This was done by modelling and simulating cases in which said techniques were incorporated. The data was later used to estimate profitabilty of each investigated case. Results showed that the highest system performance and profitability was achieved by installing bifacial modules on the site. One-axis trackers are currently too expensive, require higher maintenance and has a higher demand for land, resulting in its profitability not being justified. It was also found that the current configuration could be optimized further for higher profit, by slightly reducing the pitch as well as increasing the tilt of the existing modules. The study should be followed up by further investigating the use of backtracking for one-axis trackers. Said innovations should also be more established in the Swedish market so that CAPEX- and OPEX prices become more accessible. PV solar cells solar park bifacial solar tracker backtracking PPA NPV albedo PVsyst pitch shadowing solceller solcellspark bifacial solföljare backtracking PPA NNV albedo PVsyst radavstånd skuggning Energy Systems Energisystem

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