Activin is a dimeric protein of inhibin beta subunit, which is abundantly stored in normal bone matrix, presumably produced by osteoblasts in the process of normal bone formation. The expression of activins was examined in the wound healing of diabetic rats. In this study,insulin-dependent diabetes mellitus was induced in a group of mature Sprague-Dawley rats by injecting streptozotocin. Control animals were injected with citrate buffer only. After 3 weeks,all of rats underwent extraction of the right maxillary molars teeth after anesthesia. Rats were killed at varying intervals and the maxilla and calvaria were recovered in continuity. Tissue sections were stained with hematoxylin-eosin as well as immunohistochemical gent. Hematoxylin-eosin analyses showed that at 7 days after tooth extraction in the control and insulin-streptozotocin-treated rats there were, thick collagen fibers which formed a pretrabecular the scaffold dictated the direction of the forming trabeculae. However,the collagen fibers in the diabetic socket were thin and scanty, and only formed a narrow layer in the apical part of the socket. These histologic observations suggest that in uncontrolled, insulin-dependent diabetes, the formation of the collagenous framework in the tooth extraction socket is inhibited, resulting in delayed healing.The immunohistochemical analyses showed that at 7 days after tooth extraction in both control and insulin-streptozotocin-treated rats, osteoblasts were increased in extra-alveolar bone formation.Our findings also suggested that activin was actively involve in bone modeling during osteogenesis. These findings suggest that activin may play important role in the regulation of bone formation and it may be useful in the future for the wound healing in diabetic patients.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0731105-184112 |
| Date | 31 July 2005 |
| Creators | Tsai, Chiung-mei |
| Contributors | none, none, none, none |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0731105-184112 |
| Rights | not_available, Copyright information available at source archive |
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