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The characteristics of Neural stem cell cultured from the tilapia, Oreochromis mossambicus.

The structure and function of brain shows sexual dimorphism in the vertebrates. Sexual differentiation is divided into brain sexual differentiation and gonad sexual differentiation. Brain sexual differentiation is resulted from the neural development. In the present study, the neurosphere cloned from tilapia, Oreochromis mossambicus, was used. The characteristics of neurosphere derived from both primary- and sub- culture, were studied. The effects of epidermal growth factor (EGF), basic fibroblast growth factor (bFGF, FGF2), and temperature on the neurosphere cloned from both primary culture and subculture, were investigated. These results show that the neurospheres, cloned form both primary- and sub- culture, is consist of the nestin-immunoreactive cell. Furthermore, the cell of the neurosphere shows an ability of differentiation. And the diameter of neurosphere in the subculture is significantly larger than that of primary culture. On the other hand, both FGF and temperature have an effect to increase the diameter of neurosphere in the primary culture.

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0901111-005212
Date01 September 2011
CreatorsYang, Chu-hsien
ContributorsJuei-tang Cheng, Chin-lin Tsai, Li-hsueh Wang
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageCholon
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0901111-005212
Rightsuser_define, Copyright information available at source archive

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