This research studied the dietary effect of arachidonic acid (ARA) on growth performance and immune responses in juvenile grouper (Epinephelus coioides). The test diets used casein as protein source, and contained 9 g/100g lipid. Cod liver oil and arachidonic acid oil were used to adjust the ARA levels to 0, 0.2, 0.4, 0.6, 0.6, 0.8, and 1.0 g/100 g diet in the 5 test diets, respectively. Result of 12-week feeding trial show that the ARA supplementation had no significantly effect on the weight gain of the grouper, although 0.6 % and 0.8% groups showed higher weight gains than other groups. Liver fatty acid composition of the groupers was affected by dietary ARA. Liver ARA concentration, ARA/EPA and ARA/DHA increased with increasing dietary ARA levels. The immune responses was affected significantly by the dietary treatments. Dietary supplementation of ARA significantly enhanced plasma lysozyme activity. Lysozyme activity was increased with increasing dietary ARA levels. Dietary ARA significantly also enhanced head-kidney leucocytes respiratory burst activity. The respiratory burst activity of the 0.6 % ARA group was significantly higher than the other groups. Dietary ARA significantly enhanced head-kidney leucocyte proliferation and the dosages of 0.6 % and 0.8 % ARA were significantly more effective than the other groups. The blood phagocytic activity of the 0.6 % ARA was significantly higher than the other groups. The blood cytokine IL-1£] and TNF-£\ was significantly higher in the 0.8 % ARA group than the other groups. This research shows that feeding the grouper diets cointaining ARA at an optimal level could significantly increase immune responses of the fish.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0907111-144944 |
| Date | 07 September 2011 |
| Creators | Wu, Shih-Ting |
| Contributors | Shi-Yen Shiau, Houng-Yung Chen, Feng-Cheng Wu, Chen-Chih Kao |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0907111-144944 |
| Rights | unrestricted, Copyright information available at source archive |
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