An enzyme-linked immunosorbent assay was developed to
measure specific antibody response in bovine sera to bovine
adenovirus type 3 (BA3), an etiologic agent of respiratory
disease causing economic losses annualy to the cattle
industry. Observed endpoint titers were determined using
the intersection point from optical density values of
serially diluted sera with a positive-negative threshold.
Regression equations were determined from standards with
titers ranging from low to high and used to predict ELISA
titers from a single-serum dilution. A near-linear
relationship existed between the observed and predicted
ELISA titers of 118 bovine sera (r=0.9261). Predicted ELISA
titers were determined using the single-dilution method for
another 76 bovine sera and the correlation between the ELISA
titers and serum-virus neutralization titers for these sera
indicated a strong linear trend (r=0.8172).
Both the ELISA and serum-virus neutralization titers on
the bovine sera tested indicated widespread exposure to
several types of bovine adenovirus. Although detection of
active infection would still require examination of sera
over time for evidence of a rising titer, the single-dilution
ELISA devised should provide a rapid and sensitive
method for detection of antibody response to bovine
adenovirus type 3. / Graduation date: 1992
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/37407 |
| Date | 26 November 1991 |
| Creators | Whipple, Margaret Jo |
| Contributors | Mattson, Donald E. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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