Long segment tracheal obstruction is a potentially fatal condition arising from primary malignancy, congenital tracheal stenosis, or benign strictures. Shorter lesions are amenable to primary surgical repair, but anastomotic tension remains a limiting factor. Attention has therefore turned towards tissue engineered interposition grafts, consisting of cartilage, vascular, and airway epithelial cell layers. For clinical applications, it is essential to develop tools for noninvasive monitoring of in vivo graft development. This investigation therefore sough to optimize a tracheal patch and tube model suitable for SCID mouse implantation, and to demonstrate simultaneous tracking of human chondrocytes and epithelial cells labeled with contrasting intracellular magnetic resonance labels. Cartilage labeled with iron oxide nanoparticles was successfully distinguished from unlabeled control tissue both in vitro and in vivo. Gadolinium dendrimer labeling of adjacent airway epithelial cells, however, did not initially permit enhanced visualization. Further model optimization is required for simultaneous cell tracking.
| Identifer | oai:union.ndltd.org:YALE_med/oai:ymtdl.med.yale.edu:etd-04112010-190050 |
| Date | 30 September 2010 |
| Creators | Erickson, Benjamin Peter |
| Contributors | John Geibel, M.D., Christopher Breuer, M.D. |
| Publisher | Yale University |
| Source Sets | Yale Medical student MD Thesis |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://ymtdl.med.yale.edu/theses/available/etd-04112010-190050/ |
| Rights | restricted, I hereby grant to the Yale School of Medicine the non-exclusive license to photocopy, archive and make accessible, under the conditions specified below, my print and electronic thesis, in whole or in part, in all forms of media. <p> I agree that the Yale School of Medicine may electronically store, copy or translate my thesis to any medium or format for the purpose of preservation and accessibility. The Yale School of Medicine is not under obligation to reproduce or display my thesis in the same format in which it was originally deposited. <p> I retain all ownership rights to the thesis, including but not limited to the right to use in future works (such as articles and books) all or part of this thesis. |
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