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Degeneration of articular cartilage and cartilage healing in rabbit models.

by Linda, Fu Lap Kun. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 115-128). / ACKNOWLEDGMENT --- p.i / TABLE OF CONTENTS --- p.ii / ABSTRACT --- p.xii / Chapter CHAPTER ONE: --- INTRODUCTION --- p.1 / Chapter CHAPTER TWO : --- LITERATURE REVIEW --- p.6 / Chapter 2.1 --- BIOLOGY OF ARTICULAR CARTILAGE --- p.7 / Chapter 2.1.1 --- Normal cartilage --- p.7 / Chapter 2.1.1.1 --- Composition and properties --- p.7 / Chapter 2.1.1.2 --- Morphology of normal cartilage --- p.12 / Chapter 2.1.2 --- Degeneration of cartilage --- p.12 / Chapter 2.1.2.1 --- Morphology of degenerative cartilage --- p.12 / Chapter 2.1.2.2 --- Biochemical characteristics of degenerative cartilage --- p.13 / Chapter 2.1.3 --- Healing of degenerated articular cartilage --- p.14 / Chapter 2.2 --- METHODS TO CREATE OSTEOARTHRITIS MODEL --- p.15 / Chapter 2.2.1 --- Immobilization --- p.16 / Chapter 2.2.2 --- Instability method --- p.16 / Chapter 2.3 --- SCORING OF OSTEOARTHRITIS --- p.17 / Chapter 2.3.1 --- Gross morphology --- p.17 / Chapter 2.3.1.1 --- Mapping of OA area --- p.17 / Chapter 2.3.1.2 --- Amount of synovial fluid --- p.18 / Chapter 2.3.1.3 --- Mobility --- p.18 / Chapter 2.3.2 --- Histological scoring --- p.18 / Chapter 2.3.3 --- Biochemical comparison --- p.21 / Chapter 2.3.3.1 --- Water content --- p.21 / Chapter 2.3.3.2 --- Proteoglycan --- p.21 / Chapter 2.4 --- THE TREATMENT OF OSTEOARTHRITIS --- p.23 / Chapter 2.4.1 --- Biology of Hyaluronic acid --- p.24 / Chapter 2.4.2 --- Repairing properties of Hyaluronic acid in OA --- p.24 / Chapter 2.4.3 --- The pharmacology of Hyalgan --- p.26 / Chapter 2.4.4 --- Intraarticular injection of Hyaluronic acid in animals --- p.26 / Chapter CHAPTER THREE: --- CREATING OSTEOARTHRITIC MODEL --- p.28 / Chapter 3.1 --- INTRODUCTION --- p.29 / Chapter 3.2 --- MATERIAL AND METHODOLOGY --- p.30 / Chapter 3.2.1 --- Animals --- p.30 / Chapter 3.2.2 --- Instabilization --- p.32 / Chapter 3.2.2.1 --- Reagent and Apparatus --- p.32 / Chapter 3.2.2.2 --- Procedure --- p.32 / Chapter 3.2.3 --- Immobilization --- p.36 / Chapter 3.2.3.1 --- Reagent and Apparatus --- p.36 / Chapter 3.2.3.2 --- Procedure --- p.37 / Chapter 3.3 --- METHODS OF ASSESSMENT --- p.39 / Chapter 3.3.1 --- Gross structure comparison --- p.40 / Chapter 3.3.1.1 --- Gross mapping of the OA areas --- p.40 / Chapter 3.3.2 --- Histochemical scoring --- p.40 / Chapter 3.3.2.1 --- Procedure --- p.40 / Chapter 3.3.2.1.1 --- Hematoxylin & eosin stain --- p.45 / Chapter 3.3.2.1.2 --- Safranin O stain --- p.45 / Chapter 3.3.3 --- Biochemical assessment --- p.47 / Chapter 3.3.3.1 --- Water content --- p.47 / Chapter 3.3.3.2 --- Proteoglycan content --- p.49 / Chapter 3.3.3.2.1 --- Reagent --- p.49 / Chapter 3.3.3.2.2 --- Procedure --- p.49 / Chapter 3.3.3.2.3 --- Standard curve --- p.52 / Chapter 3.4 --- METHODS OF STATISTICAL ANALYSIS --- p.53 / Chapter 3.5 --- RESULTS --- p.54 / Chapter 3.5.1 --- Gross morphology --- p.54 / Chapter 3.5.1.1 --- General appearance --- p.54 / Chapter 3.5.1.2 --- Gross osteoarthritic area --- p.56 / Chapter 3.5.2 --- Histological results --- p.59 / Chapter 3.5.2.1 --- Histological appearance --- p.59 / Chapter 3.5.3 --- Results of biochemical assay --- p.66 / Chapter 3.3.3.1 --- Water content --- p.66 / Chapter 3.5.3.2 --- Proteoglycan --- p.69 / Chapter 3.6 --- DISCUSSION --- p.72 / Chapter 3.6.1 --- Relation between gross OA area & Mankin's score --- p.72 / Chapter 3.6.2 --- Relation between gross OA area & GAG content --- p.74 / Chapter 3.6.3 --- Relation between Mankin's score & water content --- p.76 / Chapter 3.6.4 --- Relation between Mankin's score & GAG content --- p.78 / Chapter 3.6.5 --- Comparison of the four different parameters --- p.79 / Chapter 3.6.6 --- Merit on the four parameters --- p.84 / Chapter 3.6.6 --- Consistency & reproducible of the OA model --- p.85 / Chapter 3.6.7 --- Moderately severe OA model was needed in Part II --- p.86 / Chapter 3.7 --- CONCLUSION --- p.86 / Chapter CHAPTER FOUR: --- HEALING OF CARTILAGE --- p.87 / Chapter 4.1 --- INTRODUCTION --- p.88 / Chapter 4.2 --- MATERIALS AND METHODS --- p.89 / Chapter 4.2.1 --- Establishing the osteoarthritis --- p.89 / Chapter 4.2.2 --- Treatment of the rabbits --- p.90 / Chapter 4.2.2.1 --- Reagent and Apparatus --- p.90 / Chapter 4.2.2.2 --- Procedure --- p.91 / Chapter 4.2.3 --- Methods of assessment --- p.92 / Chapter 4.2.4 --- Methods of statistical analysis --- p.92 / Chapter 4.3 --- RESULTS --- p.93 / Chapter 4.3.1 --- Gross OA area --- p.94 / Chapter 4.3.2 --- Mankin's score --- p.97 / Chapter 4.3.3 --- Water content --- p.99 / Chapter 4.3.4 --- Proteoglycan . . --- p.101 / Chapter 4.3.5 --- Results of Part I and Part II --- p.103 / Chapter 4.4 --- DISCUSSION --- p.107 / Chapter 4.4.1 --- Morphological comparison --- p.107 / Chapter 4.4.2 --- Biochemical comparison --- p.110 / Chapter 4.4.3 --- Comparison of the results in two parts of experiment --- p.111 / Chapter 4.5 --- CONCLUSION --- p.113 / BIBLIOGRAPHY --- p.114 / APPENDIX --- p.129

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_319250
Date January 1993
ContributorsFu, Lap Kun Linda., Chinese University of Hong Kong Graduate School. Division of Clinical and Pathological Sciences.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xiii, 132 leaves : ill. (chiefly mounted col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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