Cao Hui. / Publication date from spine. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves 180-194). / Acknowledgments --- p.v / Abstract --- p.vii / List of Tables --- p.xv / List of Figures --- p.xvii / Abbreviations and symbols --- p.xx / Chapter Chapter 1. --- General introduction / Chapter 1.1. --- Historical background --- p.1 / Chapter 1.2. --- Pharmacognostical development --- p.2 / Chapter 1.3. --- Importance of herb authentication --- p.3 / Chapter 1.4. --- Objective of study --- p.5 / Chapter Chapter 2. --- Literature review / Chapter 2.1. --- Botanical and taxonomic aspects --- p.9 / Chapter 2.1.1. --- Morphology --- p.9 / Chapter 2.1.2. --- Scientific names --- p.11 / Chapter 2.2. --- Chemical aspects --- p.13 / Chapter 2.3. --- Pharmacological aspects --- p.14 / Chapter 2.3.1. --- Antibacterial effect --- p.14 / Chapter 2.3.2. --- Antiphlogistic effect --- p.14 / Chapter 2.3.3. --- Antipyretic effect --- p.15 / Chapter 2.3.4. --- Effect in gastrointestinal propulsion --- p.15 / Chapter 2.3.5. --- Antineoplastic activity --- p.15 / Chapter 2.3.6. --- Hepatoprotective effect --- p.15 / Chapter 2.3.7. --- Inhibitory activity on enzymes --- p.17 / Chapter 2.3.8. --- Cardiovascular effect --- p.17 / Chapter 2.3.9. --- Acute toxicity (LD50) --- p.18 / Chapter 2.4. --- Pharmacognostical aspects --- p.18 / Chapter Chapter 3. --- Kudidan in Ben-cao literature / Chapter 3.1. --- Introduction --- p.23 / Chapter 3.2. --- Name evolution --- p.23 / Chapter 3.3. --- Natural distribution --- p.24 / Chapter 3.4. --- Characteristics --- p.25 / Chapter 3.5. --- Substitutions investigation --- p.26 / Chapter 3.6. --- Summary --- p.29 / Chapter Chapter 4. --- Morphological differences / Chapter 4.1. --- Plant identification --- p.36 / Chapter 4.1.1. --- Introduction --- p.36 / Chapter 4.1.2. --- Collection of voucher materials --- p.36 / Chapter 4.1.3. --- Plant morphology --- p.36 / Chapter 4.2. --- Macroscopical identification --- p.46 / Chapter 4.2.1. --- Introduction --- p.46 / Chapter 4.2.2. --- Materials and methods --- p.46 / Chapter 4.2.2.1. --- Commercial samples --- p.46 / Chapter 4.2.2.2. --- Macroscopical characteristics --- p.46 / Chapter 4.2.3. --- Results --- p.49 / Chapter Chapter 5. --- Histological identification / Chapter 5.1. --- Introduction --- p.58 / Chapter 5.2. --- Materials and methods --- p.59 / Chapter 5.2.1. --- Commercial samples --- p.59 / Chapter 5.2.1.1. --- Kudidan --- p.59 / Chapter 5.2.1.2. --- Pugongying --- p.59 / Chapter 5.2.1.3. --- Substitutes --- p.59 / Chapter 5.2.2. --- Authentic plant materials for comparison --- p.60 / Chapter 5.2.3. --- Methods --- p.60 / Chapter 5.2.3.1. --- Paraffin method --- p.60 / Chapter 5.2.3.2. --- Light microscopy --- p.62 / Chapter 5.2.3.3. --- Quantitative microscopy --- p.63 / Chapter 5.2.3.4. --- Scanning electron microscopy --- p.64 / Chapter 5.3. --- Results --- p.64 / Chapter 5.3.1. --- Microscopical characters of comparative plants --- p.64 / Chapter 5.3.2. --- Internal structures of herbs --- p.83 / Chapter 5.4. --- Discussion --- p.83 / Chapter Chapter 6. --- Chemical analysis / Chapter 6.1. --- Introduction --- p.99 / Chapter 6.2. --- Materials and methods --- p.100 / Chapter 6.2.1. --- Authentic samples --- p.100 / Chapter 6.2.2. --- Commercial samples --- p.100 / Chapter 6.2.3. --- Methods --- p.100 / Chapter 6.2.3.1. --- Isolation and characterization of standard substances --- p.100 / Chapter 6.2.3.2. --- Extraction of plant materials --- p.102 / Chapter 6.2.3.3. --- Thin layer chromatography --- p.102 / Chapter 6.3. --- Results and discussion --- p.104 / Chapter 6.3.1. --- TLC synopsis --- p.104 / Chapter 6.3.2. --- TLC analysis --- p.105 / Chapter 6.3.2.1. --- Qualitative evaluation of authentic plants --- p.105 / Chapter 6.3.2.2. --- Qualitative evaluation of commercial samples --- p.107 / Chapter 6.4. --- Summary --- p.107 / Chapter Chapter 7. --- Molecular fingerprinting / Chapter 7.1. --- Introduction --- p.115 / Chapter 7.2. --- Materials and methods --- p.120 / Chapter 7.2.1. --- Plant materials --- p.121 / Chapter 7.2.2. --- Herbal materials --- p.121 / Chapter 7.2.3. --- Total genomic DNA preparation --- p.121 / Chapter 7.2.3.1. --- CsCl/EtBr gradient method --- p.121 / Chapter 7.2.3.2. --- CTAB/CsCl gradient method --- p.123 / Chapter 7.2.3.3. --- CTAB miniprep method --- p.124 / Chapter 7.2.4. --- Qualitative analysis of genomic DNA --- p.125 / Chapter 7.2.5. --- Quantitative analysis of genomic DNA --- p.126 / Chapter 7.2.6. --- Genomic DNA fingerprinting --- p.126 / Chapter 7.2.6.1. --- DNA amplification --- p.126 / Chapter 7.2.6.1.1. --- AP-PCR --- p.127 / Chapter 7.2.1.1.2. --- RAPD --- p.128 / Chapter 7.2.6.2. --- Data analysis --- p.129 / Chapter 7.3. --- Results --- p.129 / Chapter 7.3.1. --- Studies on extraction of genomic DNA --- p.129 / Chapter 7.3.2. --- Genomic DNA fingerprinting by AP-PCR --- p.130 / Chapter 7.3.3. --- Genomic DNA fingerprinting by RAPD --- p.131 / Chapter 7.4. --- Discussion --- p.131 / Chapter 7.4.1. --- DNA extraction --- p.132 / Chapter 7.4.2. --- DNA fingerprinting of Kudidan --- p.136 / Chapter 7.4.3. --- Phylogenetic relationship between two genera Elephantopus and Pseudo-elephantopus of by DNA fingerprinting --- p.141 / Chapter Chapter 8. --- General summary and conclusion / Chapter 8.1. --- General summary --- p.165 / Chapter 8.1.1. --- Ben-cao investigation --- p.166 / Chapter 8.1.2. --- Investigation of commercial samples --- p.166 / Chapter 8.1.3. --- Histological characteristics --- p.167 / Chapter 8.1.4. --- Chemical analysis --- p.168 / Chapter 8.1.5. --- DNA fingerprinting --- p.168 / Chapter 8.2. --- Conclusion --- p.169 / Appendices / Chapter A) --- Solutions --- p.171 / Chapter B) --- Chinese characters cited in this Thesis --- p.173 / Chapter a) --- Herbal names --- p.173 / Chapter b) --- Book names --- p.175 / Chapter c) --- Personal names --- p.176 / Chapter d) --- Place names --- p.177 / Chapter e) --- Miscellaneous names --- p.179 / Bibliography --- p.180
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_321651 |
| Date | January 1996 |
| Contributors | Cao, Hui., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xxi, 194 leaves : ill. (some col., some mounted) ; 30 cm. |
| Coverage | China, China |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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