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Stimulus-response coupling of prostaglandin receptors in neutrophils.

by Zhi-Hui Xie. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 214-244). / Abstract --- p.i / Acknowledgments --- p.iv / Publications --- p.v / Abbreviations --- p.vi / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Neutrophils --- p.1 / Chapter 1.1.1 --- General --- p.1 / Chapter 1.1.2 --- HL-60 cells as model of neutrophils --- p.3 / Chapter 1.1.3 --- Physiological role --- p.6 / Chapter 1.1.4 --- Intracellular signalling --- p.12 / Chapter 1.1.4.1 --- General --- p.12 / Chapter 1.1.4.2 --- Intracellular signalling linked by FMLP receptors --- p.13 / Chapter 1.1.4.3 --- Intracellular signalling of neutrophil functions --- p.17 / Chapter 1.2 --- Neutrophil aggregation --- p.21 / Chapter 1.2.1 --- General --- p.21 / Chapter 1.2.2 --- Measurement of aggregation --- p.24 / Chapter 1.3 --- Prostaglandins/Prostanoids --- p.25 / Chapter 1.3.1 --- General --- p.25 / Chapter 1.3.2 --- Prostanoid receptors --- p.27 / Chapter 1.3.2.1 --- Agonists and antagonists --- p.28 / Chapter 1.3.2.2 --- EP1 receptors --- p.32 / Chapter 1.3.2.3 --- EP2 receptors --- p.33 / Chapter 1.3.2.4 --- EP3 receptors --- p.34 / Chapter 1.3.2.5 --- EP4 receptors --- p.37 / Chapter 1.3.2.6 --- DP receptors --- p.38 / Chapter 1.3.2.7 --- IP receptors --- p.39 / Chapter 1.3.2.8 --- TP receptors --- p.40 / Chapter 1.3.2.9 --- FP receptors --- p.40 / Chapter 1.4 --- Regulation of neutrophil functions by prostanoids --- p.42 / Chapter 1.4.1 --- Inhibition --- p.42 / Chapter 1.4.2 --- Activation --- p.46 / Chapter 1.4.3 --- Role of cyclic AMP --- p.48 / Chapter 1.5 --- Aim of this study --- p.51 / Chapter Chapter 2 --- Materials and methods --- p.53 / Chapter 2.1 --- Materials and solutions --- p.53 / Chapter 2.1.1 --- Materials --- p.53 / Chapter 2.1.2 --- "Buffers, solutions and cell culture medium" --- p.62 / Chapter 2.2 --- Methods --- p.64 / Chapter 2.2.1 --- Culture of HL-60 cells --- p.64 / Chapter 2.2.2 --- Differentiation of HL-60 cells --- p.65 / Chapter 2.2.3 --- Preparation of neutrophils --- p.65 / Chapter 2.2.4 --- Measurement of neutrophil aggregation --- p.67 / Chapter 2.2.5 --- Measurement of [Ca2+]i --- p.69 / Chapter 2.2.6 --- Microscopic observation --- p.70 / Chapter 2.2.7 --- Measurement of [3H]-cyclic AMP accumulation --- p.72 / Chapter 2.2.8 --- Measurement of [3H]-PGE2 receptor binding --- p.73 / Chapter 2.3 --- Data analysis --- p.76 / Chapter Chapter 3 --- Differentiation of HL-60 cells --- p.77 / Chapter 3.1 --- Introduction --- p.77 / Chapter 3.2 --- Results and discussion --- p.78 / Chapter 3.2.1 --- The observation of cell proliferation and morphology --- p.78 / Chapter 3.2.2 --- The response of HL-60 cells to FMLP --- p.80 / Chapter 3.2.3 --- The response of HL-60 cells to ATP --- p.83 / Chapter 3.3 --- Conclusion --- p.84 / Chapter Chapter 4 --- Activation of dHL-60 cells by PGE2 --- p.90 / Chapter 4.1 --- Effect of PGE2 on cell aggregation and [Ca2+]i --- p.90 / Chapter 4.1.1 --- Introduction --- p.90 / Chapter 4.1.2 --- Results --- p.90 / Chapter 4.1.2.1 --- Effect of PGE2 on cell aggregation --- p.90 / Chapter 4.1.2.2 --- Effect of PGE2 on [Ca2+]i --- p.91 / Chapter 4.1.2.3 --- Effect of PGE2 on human neutrophils --- p.93 / Chapter 4.1.2.4 --- Do PGE2 and PGE1 act at the same receptor? --- p.94 / Chapter 4.1.3 --- Discussion --- p.95 / Chapter 4.1.3.1 --- Activation of dHL-60 cells by PGE2 --- p.95 / Chapter 4.1.3.2 --- Effect of PGE2 on human neutrophils --- p.99 / Chapter 4.2 --- What does the aggregation response mean? --- p.101 / Chapter 4.2.1 --- Introduction --- p.101 / Chapter 4.2.2 --- Results --- p.101 / Chapter 4.2.2.1 --- Observation by light microscopy --- p.101 / Chapter 4.2.2.2 --- Observation by Scanning Electron Microscope --- p.102 / Chapter 4.2.3 --- Discussion --- p.103 / Chapter 4.3 --- Conclusion --- p.106 / Chapter Chapter 5 --- Characterisation of prostanoid receptors on dHL-60 cells --- p.121 / Chapter 5.1 --- Introduction --- p.121 / Chapter 5.2 --- Results --- p.121 / Chapter 5.2.1 --- Effect of prostanoid receptor agonists and antagonists on cell aggregation --- p.121 / Chapter 5.2.2 --- Effect of prostanoid receptor agonists on [Ca2+]i --- p.122 / Chapter 5.3 --- Discussion --- p.123 / Chapter 5.3.1 --- Involvement of prostanoid receptors --- p.123 / Chapter 5.3.2 --- Involvement of EP receptors --- p.126 / Chapter 5.4 --- Conclusion --- p.130 / Chapter Chapter 6 --- Binding studies of [3H]-PGE2 on rat neutrophils and dHL-60 cells --- p.137 / Chapter 6.1 --- Introduction --- p.137 / Chapter 6.2 --- Results --- p.138 / Chapter 6.3 --- Discussion --- p.140 / Chapter Chapter 7 --- The mechanism of action of PGE2 in activating dHL-60 cells --- p.146 / Chapter 7.1 --- Introduction --- p.146 / Chapter 7.2 --- Methods --- p.147 / Chapter 7.2.1 --- Pretreatment with enzyme inhibitors --- p.147 / Chapter 7.2.2 --- Pretreatment with toxins --- p.148 / Chapter 7.2.3 --- Pretreatment with PMA --- p.148 / Chapter 7.3 --- Results and discussion --- p.148 / Chapter 7.3.1 --- The role of Ca2+ --- p.149 / Chapter 7.3.2 --- The role of cyclic AMP --- p.152 / Chapter 7.3.3 --- The role of PI3-kinase --- p.159 / Chapter 7.3.4 --- The role of PLD --- p.165 / Chapter 7.3.5 --- The role of PLA2 --- p.169 / Chapter 7.3.6 --- The role of tyrosine kinase --- p.171 / Chapter 7.3.7 --- The role of PKC --- p.173 / Chapter 7.3.8 --- The role of G protein --- p.180 / Chapter 7.3.9 --- The role of cyclic GMP --- p.185 / Chapter 7.3.10 --- The role of KATP channels --- p.186 / Chapter 7.4 --- Conclusion --- p.187 / Chapter Chapter 8 --- General discussion and conclusion --- p.209 / References --- p.214

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_321692
Date January 1996
ContributorsXie, Zhi-Hui., Chinese University of Hong Kong Graduate School. Division of Basic Medical Sciences.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xi, 244 leaves : ill. (some col., some mounted) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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